PDF(Pubmed)
Abstract：
Environmental contamination and the resulting food contamination represent a serious problem and pose a major threat to animal and human health. The gastrointestinal tract is directly exposed to a variety of substances. One is glyphosate, whose presence in the soil is commonly observed. This study demonstrates the effects of low and high glyphosate doses on the populations of intramural neurons of the porcine descending colon. An analysis was performed on neurons ex-pressing the vasoactive intestinal peptide, pituitary adenylate cyclase-activating peptide, a neuronal isoform of nitrogen oxide synthase, and galanin. Even a low dose of glyphosate increased the number of neurons immunoreactive against the studied substances. However, the changes depended on both the plexus analysed and the substance tested. Meanwhile, a high glyphosate dose resulted in quantitative changes (an increase in the number) within neurons immunoreactive against all the studied neuropeptides/enzymes in the myenteric plexus and both submucosal plexuses. The response of the enteric nervous system in the form of an increase in the number of neurons immunoreactive against neuroprotective substances may suggest that glyphosate has a toxic effect on enteric neurons which attempt to increase their survivability through the released neuroprotective substances.
摘要：
环境污染和由此产生的食品污染是一个严重的问题，对动物和人类健康构成重大威胁。胃肠道直接暴露于各种物质。一种是草甘膦，它在土壤中的存在通常被观察到。这项研究证明了低和高草甘膦剂量对猪降结肠壁内神经元群体的影响。对表达血管活性肠肽的神经元进行了分析，垂体腺苷酸环化酶激活肽，一氧化氮合酶的神经元同工型，还有Galanin.即使低剂量的草甘膦也会增加对所研究物质具有免疫反应性的神经元的数量。然而,这些变化取决于所分析的神经丛和所测试的物质。同时,高草甘膦剂量导致对肌间神经丛和两个粘膜下神经丛中所有研究的神经肽/酶免疫反应性的神经元内的定量变化（数量增加）。肠神经系统以对神经保护物质具有免疫反应性的神经元数量增加的形式的反应可能表明草甘膦对肠神经元具有毒性作用，肠神经元试图通过释放的神经保护物质增加其生存能力。
