PDF(Pubmed)
Abstract：
Epigenetic aging is a hot topic in the field of aging research. The present study estimated epigenetic age in long-lived individuals, who are currently actively being studied worldwide as an example of successful aging due to their longevity. We used Bekaert\'s blood-based age prediction model to estimate the epigenetic age of 50 conditionally \"healthy\" and 45 frail long-livers over 90 years old. Frailty assessment in long-livers was conducted using the Frailty Index. The control group was composed of 32 healthy individuals aged 20-60 years. The DNA methylation status of 4 CpG sites (ASPA CpG1, PDE4C CpG1, ELOVL2 CpG6, and EDARADD CpG1) included in the epigenetic clock was assessed through pyrosequencing. According to the model calculations, the epigenetic age of long-livers was significantly lower than their chronological age (on average by 21 years) compared with data from the group of people aged 20 to 60 years. This suggests a slowing of epigenetic and potentially biological aging in long livers. At the same time, the obtained results showed no statistically significant differences in delta age (difference between the predicted and chronological age) between \"healthy\" long livers and long livers with frailty. We also failed to detect sex differences in epigenetic age either in the group of long livers or in the control group. It is possible that the predictive power of epigenetic clocks based on a small number of CpG sites is insufficient to detect such differences. Nevertheless, this study underscores the need for further research on the epigenetic status of centenarians to gain a deeper understanding of the factors contributing to delayed aging in this population.
摘要：
表观遗传衰老是衰老研究领域的热点。本研究估计了长寿个体的表观遗传年龄，他们目前正在全球范围内积极研究，作为由于他们的长寿而成功衰老的一个例子。我们使用Bekaert的基于血液的年龄预测模型来估计50个有条件的“健康”和45个90岁以上的体弱长肝的表观遗传年龄。使用脆弱指数进行长期肝脏的脆弱评估。对照组由32名20-60岁的健康个体组成。通过焦磷酸测序评估表观遗传钟中包含的4个CpG位点（ASPACpG1，PDE4CCpG1，ELOVL2CpG6和EDARADDCpG1）的DNA甲基化状态。根据模型计算,与20~60岁人群的数据相比,长肝的表观遗传年龄显著低于实际年龄(平均21岁).这表明在长肝中表观遗传和潜在的生物衰老的减缓。同时,获得的结果表明，“健康”长肝和虚弱的长肝之间的年龄差异没有统计学意义（预测年龄和实际年龄之间的差异）。我们也未能在长肝组或对照组中检测到表观遗传年龄的性别差异。基于少量CpG位点的表观遗传钟的预测能力可能不足以检测这种差异。然而，这项研究强调需要对百岁老人的表观遗传状况进行进一步研究,以更深入地了解导致该人群衰老延迟的因素.
