Abstract：
Valeriana jatamansi Jones is a commonly used traditional Chinese medicine, boasting rich effective compositions with versatile chemical structures and wide polarity, including iridoids, chlorogenic acid, and flavonoids. Previous reports indicate that conventional high-performance liquid chromatography (HPLC) analytical methods have proven inefficient performance in comprehensively characterizing components in Valeriana jatamansi. In the present study, a hybrid online analytical platform combining supercritical fluid extraction with both conventional HPLC separation (reverse phase) and supercritical fluid chromatography (normal phase) has been established and validated. This system can provide online extraction with two different chromatographic separation modes to increase separation ability and has been connected to a mass spectrometer to acquire high-resolution mass spectrometry data. Then, the online platform was applied to screening components in Valeriana jatamansi. A total of 117 compounds were identified, including five lignans, 18 organic acids, six flavonoids, and 88 iridoids. Thirty-three compounds were reported from Valeriana jatamansi for the first time. These results enrich our understanding of the components of Valeriana jatamansi and prove that the developed online platform in this study is a robust approach for accelerating working efficiency in comprehensively analyzing complicated samples.
摘要：
ValerianajatamansiJones是一种常用的中药，拥有丰富的有效组合物，具有多功能的化学结构和广泛的极性，包括环烯醚萜类,绿原酸,和类黄酮.先前的报道表明，传统的高效液相色谱（HPLC）分析方法已证明在全面表征Valerianajatamansi中的成分方面表现不佳。在本研究中,已建立并验证了将超临界流体萃取与常规HPLC分离（反相）和超临界流体色谱（正相）相结合的混合在线分析平台。该系统可以提供具有两种不同色谱分离模式的在线提取，以提高分离能力，并已连接到质谱仪以获取高分辨率质谱数据。然后，将该在线平台应用于Valerianajatamansi中的成分筛选.共鉴定出117种化合物,包括五种木脂素类,18种有机酸，六种类黄酮，和88个环烯醚萜类。首次从Valerianajatamansi中报道了33个化合物。这些结果丰富了我们对Valerianajatamansi组件的理解，并证明了本研究中开发的在线平台是一种可靠的方法，可加快全面分析复杂样品的工作效率。
