Abstract:
Co-fermentation of multiple substrates has emerged as the most effective method to improve the yield of bioproducts. Herein, sustainable rubberwood enzymatic hydrolysates (RWH) were co-fermented by Aureobasidium pullulans to produce poly(β-L-malic acid) (PMA), and RWH + glucose/xylose was also investigated as co-substrates. Owing to low inhibitor concentration and abundant natural nitrogen source content of RWH, a high PMA yield of 0.45 g/g and a productivity of 0.32 g/L/h were obtained by RWH substrate fermentation. After optimization, PMA yields following the fermentation of RWH + glucose and RWH + xylose reached 59.92 g/L and 53.71 g/L, respectively, which were 52 % and 36 % higher than that after the fermentation of RWH. RWH + glucose more significantly affected the correlation between PMA yield and substrate concentration than RWH + xylose. The results demonstrated that the co-fermentation of RWH co-substrate is a promising method for the synthesis of bioproducts.
摘要:
多种底物的共发酵已成为提高生物产品产量的最有效方法。在这里,可持续的橡胶木酶水解产物(RWH)由出芽梭菌共发酵产生聚(β-L-苹果酸)(PMA),还研究了RWH+葡萄糖/木糖作为共底物。由于RWH的抑制剂浓度低,天然氮源含量丰富,通过RWH底物发酵获得0.45g/g的高PMA产量和0.32g/L/h的生产率。优化后,RWH+葡萄糖和RWH+木糖发酵后PMA产量分别达到59.92g/L和53.71g/L,分别,分别比RWH发酵后提高了52%和36%。RWH+葡萄糖比RWH+木糖更显著地影响PMA产量与底物浓度之间的相关性。结果表明,RWH共底物的共发酵是合成生物制品的一种有前途的方法。