UNASSIGNED: Twenty-four New Zealand rabbits were divided into the following three groups: experimental model of coronary venous hypertension (CVH) (n = 8), sham group (n = 8), and control group (n = 8). After 21 days postoperatively, tissue samples from each group were compared for perivascular inflammation, erythrocyte extravasation, macrophage infiltration, and hemosiderin deposits by histopathological scoring under a light microscope. Matrix metalloproteinase-2 (MMP-2) activation was evaluated using immunohistochemical staining.
UNASSIGNED: In the CVH group, hemosiderin accumulation was significantly higher than in the sham and control groups (1.0 (1.0-3.0), 0.0 (0.0-1.0), 0.0 (0.0-0.0); p < 0.001). Immunohistochemically, in the CVH group, MMP-2 levels were significantly higher than in the sham and control groups (2.0 (1.0-3.0), 0.0 (0.0-1.0), 0.0 (0.0-0.0); p < 0.001).
UNASSIGNED: This experimental study showed for the first time the histopathological and immunohistochemical changes in myocardial tissue, similar to those observed in CVI, as a result of increased coronary venous pressure due to coronary vein ligation. Further studies are needed to understand the clinical implications of these results.
■将24只新西兰兔分为以下三组:冠状静脉高压(CVH)的实验模型(n=8),假手术组(n=8),对照组(n=8)。术后21天,比较各组组织样本的血管周围炎症,红细胞外渗,巨噬细胞浸润,在光学显微镜下通过组织病理学评分和铁血黄素沉积。使用免疫组织化学染色评估基质金属蛋白酶-2(MMP-2)的激活。
■在CVH组中,含铁血黄素的积累明显高于假手术组和对照组(1.0(1.0-3.0),0.0(0.0-1.0),0.0(0.0-0.0);p<0.001)。免疫组织化学,在CVH组中,MMP-2水平明显高于假手术组和对照组(2.0(1.0-3.0),0.0(0.0-1.0),0.0(0.0-0.0);p<0.001)。
■这项实验研究首次显示了心肌组织的组织病理学和免疫组织化学变化,与CVI中观察到的相似,由于冠状静脉结扎导致的冠状静脉压升高。需要进一步的研究来了解这些结果的临床意义。