PDF(Pubmed)
Abstract：
OBJECTIVE: Staphylococcus aureus is an important clinical pathogen that causes a high number of antibiotic-resistant infections. The study of S. aureus biology, and particularly of the function of essential proteins, is of particular importance to develop new approaches to combat this pathogen. We have optimized a clustered regularly interspaced short palindromic repeat interference (CRISPRi) system that allows efficient targeting of essential S. aureus genes. Furthermore, we have used that system to construct a library comprising 261 strains, which allows the depletion of essential proteins encoded by 200 genes/operons. This library, which we have named Lisbon CRISPRi Mutant Library, should facilitate the study of S. aureus pathogenesis and biology.
摘要：
目的：金黄色葡萄球菌是一种重要的临床病原菌，可引起大量耐药菌感染。金黄色葡萄球菌生物学的研究,特别是必需蛋白质的功能，对于开发对抗这种病原体的新方法特别重要。我们已经优化了成簇的规则间隔短回文重复干扰（CRISPRi）系统，该系统允许有效靶向必需的金黄色葡萄球菌基因。此外，我们已经使用该系统构建了包含261个菌株的文库，这允许消耗由200个基因/操纵子编码的必需蛋白质。这个图书馆,我们将其命名为里斯本CRISPri突变库，有助于金黄色葡萄球菌发病机制和生物学的研究。
