PDF(Pubmed)
Abstract：
Soybean cyst nematode (SCN) is a destructive pathogen of soybeans responsible for annual yield loss exceeding $1.5 billion in the United States. Here, we conducted a series of genome-wide association studies (GWASs) to understand the genetic landscape of SCN resistance in the University of Missouri soybean breeding programs (Missouri panel), as well as germplasm and cultivars within the United States Department of Agriculture (USDA) Uniform Soybean Tests-Northern Region (NUST). For the Missouri panel, we evaluated the resistance of breeding lines to SCN populations HG 2.5.7 (Race 1), HG 1.2.5.7 (Race 2), HG 0 (Race 3), HG 2.5.7 (Race 5), and HG 1.3.6.7 (Race 14) and identified seven quantitative trait nucleotides (QTNs) associated with SCN resistance on chromosomes 2, 8, 11, 14, 17, and 18. Additionally, we evaluated breeding lines in the NUST panel for resistance to SCN populations HG 2.5.7 (Race 1) and HG 0 (Race 3), and we found three SCN resistance-associated QTNs on chromosomes 7 and 18. Through these analyses, we were able to decipher the impact of seven major genetic loci, including three novel loci, on resistance to several SCN populations and identified candidate genes within each locus. Further, we identified favorable allelic combinations for resistance to individual SCN HG types and provided a list of available germplasm for integration of these unique alleles into soybean breeding programs. Overall, this study offers valuable insight into the landscape of SCN resistance loci in U.S. public soybean breeding programs and provides a framework to develop new and improved soybean cultivars with diverse plant genetic modes of SCN resistance.
摘要：
大豆胞囊线虫（SCN）是大豆的破坏性病原体，在美国造成的年产量损失超过15亿美元。这里,我们进行了一系列全基因组关联研究（GWASs），以了解密苏里大学大豆育种计划（密苏里小组）中SCN抗性的遗传景观，以及美国农业部（USDA）北部地区统一大豆测试（NUST）内的种质和品种。对于密苏里州的小组，我们评估了育种品系对SCN种群HG2.5.7（种族1）的抗性，HG1.2.5.7（比赛2），HG0（第3场比赛），HG2.5.7(第5场),和HG1.3.6.7（种族14），并在2、8、11、14、17和18号染色体上鉴定了与SCN抗性相关的七个定量性状核苷酸（QTNs）。此外,我们评估了NUST面板中的育种品系对SCN种群HG2.5.7（种族1）和HG0（种族3）的抗性，我们在7号和18号染色体上发现了三个与SCN抗性相关的QTNs。通过这些分析,我们能够破译七个主要遗传基因座的影响，包括三个新颖的基因座，对几个SCN种群的抗性，并在每个基因座内确定了候选基因。Further,我们确定了对单个SCNHG类型具有抗性的有利等位基因组合，并提供了将这些独特等位基因整合到大豆育种计划中的可用种质列表。总的来说,这项研究为美国公共大豆育种计划中SCN抗性基因座的景观提供了宝贵的见解，并提供了一个框架来开发具有SCN抗性的多种植物遗传模式的新型改良大豆品种。
