Abstract：
Due to gradual environmental changes like ozone layer depletion and global warming, human eyes are exposed to UV light. Exposure to UV light can be a cause of cataracts, one of the ocular diseases that may cause vision impairment. To date, lens replacement has been the only treatment available for cataracts. In our present study, we carried out an extensive examination of polyphenols as inhibitors for UV-induced aggregation of γD-crystallin. On exposure to UV-C light, γD-crystallin forms fibrils instead of amorphous aggregates. Various polyphenols were tested as inhibitors; out of them, quercetin, baicalein, and caffeic acid were found to be effective. As polyphenols are insoluble in water, nanoencapsulation was used to enhance their bioavailability. CS-TPP and CS-PLGA encapsulating systems were considered, as they form biodegradable nanocapsules. Out of three polyphenols (quercetin, baicalein, and caffeic acid), quercetin forms nanocarriers of smaller sizes, a must for crossing the retinal barrier. Quercetin nanocarriers were considered an effective system that could be used for therapeutic applications. For these nanocarriers, encapsulation efficiency and polyphenol release kinetics were studied. CS-PLGA NPs were found to have a better loading efficiency for quercetin than CS-TPP NPs.
摘要：
由于臭氧层消耗和全球变暖等环境的逐渐变化，人眼暴露于紫外线。暴露在紫外线下可能是白内障的原因，可能导致视力障碍的眼部疾病之一。迄今为止,更换晶状体是白内障唯一可用的治疗方法。在我们目前的研究中，我们对多酚作为紫外线诱导的γD-晶状体蛋白聚集的抑制剂进行了广泛的研究。暴露在UV-C光下,γD-晶状体蛋白形成原纤维而不是无定形聚集体。测试了各种多酚作为抑制剂；其中，槲皮素,黄芩素,咖啡酸被发现是有效的。由于多酚不溶于水，纳米封装用于提高其生物利用度。考虑了CS-TPP和CS-PLGA封装系统，当它们形成可生物降解的纳米胶囊时。三种多酚（槲皮素，黄芩素,和咖啡酸)，槲皮素形成较小尺寸的纳米载体，必须穿过视网膜屏障。槲皮素纳米载体被认为是可用于治疗应用的有效系统。对于这些纳米载体，研究了包封率和多酚释放动力学。发现CS-PLGANP比CS-TPPNP具有更好的槲皮素负载效率。
