PDF(Pubmed)
Abstract：
OBJECTIVE: Porcine epidemic diarrhea (PED) is a highly infectious and economically significant gastrointestinal disorder that affects pigs of all ages. Preventing and controlling PED is achieved by immunizing sows with vaccines, enabling passive piglet immunization via colostrum. The prevalence of G2b porcine epidemic diarrhea virus (PEDV) continues in China despite the use of commercial vaccines, raising questions regarding current vaccine efficacy and the need for novel vaccine development. Adenovirus serotype 5 (Ad5) has several advantages, including high transduction efficiency, a wide range of host cells, and the ability to infect cells at various stages. In this study, we expressed the immunogenic proteins of spike (S) using an Ad5 vector and generated a PED vaccine candidate by inducing significant humoral immunity. The rAd5-PEDV-S prevented PED-induced weight loss, diarrhea, and intestinal damage in piglets. This novel vaccine candidate strain possesses the potential for use in the pig breeding industry.
摘要：
目的：猪流行性腹泻（PED）是一种高度传染性和经济意义重大的胃肠道疾病，影响所有年龄的猪。预防和控制PED是通过用疫苗免疫母猪来实现的，通过初乳进行被动仔猪免疫。尽管使用了商业疫苗，G2b猪流行性腹泻病毒（PEDV）在中国的流行仍在继续，提出了有关当前疫苗功效和新型疫苗开发需求的问题。腺病毒血清型5（Ad5）有几个优点，包括高转导效率，广泛的宿主细胞，以及在不同阶段感染细胞的能力。在这项研究中,我们使用Ad5载体表达了spike（S）的免疫原性蛋白，并通过诱导显着的体液免疫产生了PED疫苗候选物。rAd5-PEDV-S可以预防PED引起的体重减轻，腹泻,和仔猪肠道损伤。这种新型疫苗候选株具有用于猪养殖业的潜力。
