Abstract：
Novel high-throughput protein detection technologies are critically needed for population-based large-scale SARS-CoV-2 antibody detection as well as for monitoring quality and duration of immunity against virus variants. Current protein microarray techniques rely heavily on labeled transduction methods that require sophisticated instruments and complex operations, limiting their clinical potential, particularly for point-of-care (POC) applications. Here, we developed a label-free and naked-eye readable microarray (NRM) based on a thickness-sensing plasmon ruler, enabling antibody profiling within 30 min. The NRM chips provide 100% accuracy for neutralizing antibody detection by efficiently screening antigen types and experimental conditions and allow for the profiling of antibodies against multiple SARS-CoV-2 variants in clinical samples. We further established a flexible \"barcode\" NRM assay with a simple tape-based operation, enabling an effective smartphone-based readout and analysis. These results demonstrate new strategies for high-throughput protein detection and highlight the potential of novel protein microarray techniques for realistic clinical applications.
摘要：
新型高通量蛋白质检测技术对于基于人群的大规模SARS-CoV-2抗体检测以及监测针对病毒变体的免疫质量和持续时间是至关重要的。当前的蛋白质微阵列技术严重依赖于标记的转导方法，这些方法需要复杂的仪器和复杂的操作，限制了他们的临床潜力，特别是对于即时护理（POC）应用。这里,我们开发了一种基于厚度感应等离子体标尺的无标签和肉眼可读的微阵列（NRM），在30分钟内实现抗体谱分析。NRM芯片通过有效筛选抗原类型和实验条件，为中和抗体检测提供100％的准确性，并允许在临床样品中针对多种SARS-CoV-2变体的抗体谱分析。我们进一步建立了一个灵活的“条形码”NRM测定与一个简单的基于磁带的操作，实现基于智能手机的有效读出和分析。这些结果证明了高通量蛋白质检测的新策略，并突出了新型蛋白质微阵列技术在实际临床应用中的潜力。
