Abstract：
Atopic dermatitis (AD) is a chronic, inflammatory cutaneous disease driven by immune dysregulation. Catalpol is an iridoids, possessing anti-inflammatory, antioxidant, and neuroprotective activities. It can be added to food as a dietary supplement. To evaluate the effects and mechanisms of catalpol on AD, both in vitro and in vivo studies were conducted. It was found that catalpol downregulated the phosphorylation of Lyn and Syk to inhibit various downstream pathways, including intracellular Ca2+ elevation, cytokines generation, and histamine release, which ultimately controlled mast cell (MCs) degranulation. The results showed that catalpol alleviated AD-like skin lesions and MC infiltration via regulation of pro-Th2 and Th2 cytokines in vivo. Furthermore, this compound reduced the levels of IgE in AD mice and improved allergic reactions in PCA mice. The results provided that catalpol was potentially developed as a dietary supplement to improve AD and other atopic diseases.
摘要：
特应性皮炎(AD)是一种慢性、由免疫失调引起的炎症性皮肤病。Catalpol是一种环烯醚萜类化合物,具有抗炎作用,抗氧化剂，和神经保护活动。它可以作为膳食补充剂添加到食物中。目的：评价catalol对AD的作用及其机制。进行了体外和体内研究。发现catalpol下调Lyn和Syk的磷酸化以抑制各种下游途径，包括细胞内Ca2+升高，细胞因子的产生，和组胺释放，最终控制肥大细胞（MC）脱颗粒。结果表明，在体内，catalpol通过调节pro-Th2和Th2细胞因子减轻了AD样皮肤病变和MC浸润。此外，该化合物降低了AD小鼠的IgE水平，并改善了PCA小鼠的过敏反应。该结果提供了catalpol可能被开发为改善AD和其他特应性疾病的膳食补充剂。
