DNA was extracted from the peripheral blood of patients. The Agena MassARRAY platform was used for the genotyping. Statistical analysis was conducted using the chi-squared test or Fisher\'s exact test, logistic regression analysis, and stratification analysis.
The \'G\' allele of rs989902 (PTPN13) and the \'T\' allele of rs738722 (CHEK2) were both associated with an increased risk of ESCC (rs989902: OR = 1.23, 95% CI = 1.02-1.47, p = 0.028; rs738722: OR = 1.28, 95% CI = 1.06-1.55, p = 0.011). Stratification analysis showed that SNPs (rs989902 and rs738722) were notably correlated with an increased risk of ESCC after stratification for age, sex, smoking, and drinking status. In addition, rs738722 might be associated with lower stage, while rs989902 had a lower risk of metastasis.
Our findings display that PTPN13 rs989902 and CHEK2 rs738722 are associated with an increased risk of ESCC in the Chinese Han population.
■从患者外周血中提取DNA。AgenaMassARRAY平台用于基因分型。使用卡方检验或Fisher精确检验进行统计分析,Logistic回归分析,和分层分析。
■rs989902的\'G\'等位基因(PTPN13)和rs738722的\'T'等位基因(CHEK2)均与ESCC的风险增加相关(rs989902:OR=1.23,95%CI=1.02-1.47,p=0.028;rs738722:OR=1.28-95%CI=1.55,分层分析显示,SNPs(rs989902和rs738722)与年龄分层后ESCC风险增加显著相关,性别,吸烟,和饮酒状况。此外,rs738722可能与低级相关,而rs989902具有较低的转移风险。
■我们的研究结果表明,在中国汉族人群中,PTPN13rs989902和CHEK2rs738722与ESCC风险增加相关。