PDF(Pubmed)
Abstract：
Riemerella anatipestifer (RA) is a notorious duck pathogen, characterized by a multitude of serotypes that exhibit no cross-reaction with one another. Moreover, RA is resistant to various antibacterial agents. Consequently, understanding the mechanisms behind resistance and identifying potential targets for drug development have become pressing needs. In this study, we show that the two TolC proteins play a role in the resistance to different drugs and metals and in the virulence. The results suggest that TolCA has a wider range of efflux substrates than TolCB. Except for gentamicin, neither TolCA nor TolCB was involved in the efflux of the other tested antibiotics. Strikingly, TolCA but not TolCB enhanced the frequency of resistance-conferring mutations. Moreover, TolCA was involved in RA virulence. Given its conservation in RA, TolCA has potential as a drug target for the development of therapeutics against RA infections.
摘要：
目的：鼠疫Riemerella(RA)是一种臭名昭著的鸭病原，特征在于多种血清型彼此不表现出交叉反应。此外，RA对各种抗菌剂具有抗性。因此,了解耐药性背后的机制和确定药物开发的潜在靶标已成为迫切需要。在这项研究中,我们表明这两种TolC蛋白在对不同药物和金属的抗性和毒力中起作用。结果表明，TolCA比TolCB具有更广泛的外排底物。除了庆大霉素,TolCA和TolCB均未参与其他受试抗生素的外排.引人注目的是，TolCA而不是TolCB增加了抗性突变的频率。此外，TolCA参与RA毒力。鉴于其在RA中的保守性，TolCA具有作为开发针对RA感染的治疗剂的药物靶标的潜力。
