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Abstract：
Coat color, as a distinct phenotypic characteristic of pigs, is often subject to preference and selection, such as in the breeding process of new breed. Shanxia long black pig was derived from an intercross between Berkshire boars and Licha black pig sows, and it was bred as a paternal strain with high-quality meat and black coat color. Although the coat color was black in the F1 generation of the intercross, it segregated in the subsequent generations. This study aims to decode the genetic basis of coat color segregation and develop a method to distinct black pigs from the spotted in Shanxia long black pig.
Only a QTL was mapped at the proximal end of chromosome 6, and MC1R gene was picked out as functional candidate gene. A total of 11 polymorphic loci were identified in MC1R gene, and only the c.67_68insCC variant was co-segregating with coat color. This locus isn\'t recognized by any restriction endonuclease, so it can\'t be genotyped by PCR-RFLP. The c.370G > A polymorphic locus was also significantly associated with coat color, and has been in tightly linkage disequilibrium with the c.67_68insCC. Furthermore, it is recognized by BspHI. Therefore, a PCR-RFLP method was set up to genotype this locus. Besides the 175 sequenced individuals, another more 1,391 pigs were genotyped with PCR-RFLP, and all of pigs with GG (one band) were black.
MC1R gene (c.67_68insCC) is the causative gene (mutation) for the coat color segregation, and the PCR-RFLP of c.370G > A could be used in the breeding program of Shanxia long black pig.
Only a QTL was mapped at the proximal end of chromosome 6, and MC1R gene was picked out as functional candidate gene. A total of 11 polymorphic loci were identified in MC1R gene, and only the c.67_68insCC variant was co-segregating with coat color. This locus isn\'t recognized by any restriction endonuclease, so it can\'t be genotyped by PCR-RFLP. The c.370G > A polymorphic locus was also significantly associated with coat color, and has been in tightly linkage disequilibrium with the c.67_68insCC. Furthermore, it is recognized by BspHI. Therefore, a PCR-RFLP method was set up to genotype this locus. Besides the 175 sequenced individuals, another more 1,391 pigs were genotyped with PCR-RFLP, and all of pigs with GG (one band) were black.
MC1R gene (c.67_68insCC) is the causative gene (mutation) for the coat color segregation, and the PCR-RFLP of c.370G > A could be used in the breeding program of Shanxia long black pig.
摘要：
背景：外套颜色，作为猪的独特表型特征，经常受到偏好和选择的影响，例如在新品种的育种过程中。山下长黑猪来源于伯克希尔公猪和利查黑猪母猪的杂交，它被培育为具有优质肉类和黑色外套颜色的父系。尽管在F1代交叉中，外套颜色为黑色，它在后代中隔离。本研究旨在揭示皮毛颜色分离的遗传基础，并开发一种区分山下长黑猪中黑猪和斑点的方法。
结果：只有一个QTL定位在6号染色体的近端，并挑选出MC1R基因作为功能候选基因。MC1R基因共鉴定出11个多态位点,只有c.67_68insCC变体与涂层颜色共分离。这个基因座不被任何限制性内切酶识别,所以它不能通过PCR-RFLP进行基因分型。c.370G>A多态性位点也与毛色显著相关，并与c.67_68insCC紧密连锁不平衡。此外，它被BSPHI认可。因此,建立了PCR-RFLP方法来对该基因座进行基因分型。除了175个测序的个体，用PCR-RFLP对另外1,391头猪进行了基因分型，所有GG（一条带）的猪都是黑色的。
结论：MC1R基因（c.67_68insCC）是毛色分离的致病基因（突变），c.370G>A的PCR-RFLP可用于山夏长黑猪的育种程序。
结果：只有一个QTL定位在6号染色体的近端，并挑选出MC1R基因作为功能候选基因。MC1R基因共鉴定出11个多态位点,只有c.67_68insCC变体与涂层颜色共分离。这个基因座不被任何限制性内切酶识别,所以它不能通过PCR-RFLP进行基因分型。c.370G>A多态性位点也与毛色显著相关，并与c.67_68insCC紧密连锁不平衡。此外，它被BSPHI认可。因此,建立了PCR-RFLP方法来对该基因座进行基因分型。除了175个测序的个体，用PCR-RFLP对另外1,391头猪进行了基因分型，所有GG（一条带）的猪都是黑色的。
结论：MC1R基因（c.67_68insCC）是毛色分离的致病基因（突变），c.370G>A的PCR-RFLP可用于山夏长黑猪的育种程序。
