Abstract：
Behavioral changes in zebrafish are an effective early warning system to determine water quality. However, only a few studies have examined the response of zebrafish to non-chemical stimulus after exposure to a contaminant. Therefore, this study investigated the differences in the behavioral responses of zebrafish to acoustic stimuli before and after exposure to cadmium (Cd). Acoustic escape response sensitivity curves were obtained and analyzed, followed by the determination of sensitive stimulus conditions at 100 Hz and 97 dB with a duration of 30 s and an interval of 30 min. Zebrafish exhibit a significant acoustic escape response, which is significantly reduced after exposure to Cd. The results showed that zebrafish stop demonstrating acoustic escape responses when exposed to higher Cd concentrations or longer acoustic exposures. Based on these results, a novel method for detecting abnormal behavior in zebrafish by acoustic stimulation has been proposed, which is expected to reduce the false alarm rate of this type of water quality technology.
摘要：
斑马鱼的行为变化是确定水质的有效预警系统。然而,只有少数研究检查了斑马鱼暴露于污染物后对非化学刺激的反应。因此,这项研究调查了斑马鱼在暴露于镉（Cd）之前和之后对声刺激的行为反应的差异。获得并分析了声逃逸响应灵敏度曲线,然后确定100Hz和97dB的敏感刺激条件，持续时间为30s，间隔为30分钟。斑马鱼表现出显著的声逃逸反应，暴露于Cd后显著降低。结果表明，斑马鱼在暴露于较高的Cd浓度或较长的声学暴露时停止表现出声逸出响应。基于这些结果,提出了一种通过声刺激检测斑马鱼异常行为的新方法，有望降低此类水质技术的误报率。
