PDF(Pubmed)
Abstract：
In recent years, the infection rate of avian encephalomyelitis virus (AEV) infection in chickens has risen significantly, seriously endangering the development of the chicken industry. In order to study the current epidemiological status of AEV in China as well as the genetic and evolutionary patterns of the virus, we conducted a survey and genomic analysis of chicken AEV. The results showed that 46.26% (136/294) of the tissue samples tested (n = 294) were positive for AEV, with the highest positivity rate of 62.24% (61/98) among tissue samples from chickens aged 13 to 18 wk. The complete genomes of 2 representative AEV strains were determined, and the VP1 evolutionary tree results revealed that the 2 representative strains belonged to a novel AEV strain. Multiple alignment analysis showed that the ORF1 genes of the 2 representative strains differed by 82.3 to 99.9% at the amino acid level compared with the reference AEV strain, and the mutations at the key amino acid loci of VP2 and VP3 were the same as those in the chick embryo-adapted strain. The analysis makes up for the molecular epidemiological data and genetic variation of the 2 representative strains. The analysis makes up for the molecular epidemiological data and genetic variation of AEV and provides a basis for further understanding the spread of AEV in China.
摘要：
近年来,鸡的禽脑脊髓炎病毒(AEV)感染率显著上升,严重危害养鸡业的发展。为了研究我国AEV的流行病学现状以及该病毒的遗传和进化模式,我们对鸡AEV进行了调查和基因组分析。结果显示,46.26%(136/294)的组织样本(n=294)为AEV阳性,在13至18周龄鸡的组织样本中，阳性率最高，为62.24％（61/98）。确定了2个代表性AEV菌株的完整基因组，VP1进化树结果表明，2个代表菌株属于新的AEV菌株。多重比对分析表明，与参考AEV菌株相比，2个代表菌株的ORF1基因在氨基酸水平上差异为82.3％至99.9％，VP2和VP3关键氨基酸位点的突变与适应鸡胚的菌株相同。该分析弥补了2个代表性菌株的分子流行病学数据和遗传变异。该分析弥补了AEV的分子流行病学数据和遗传变异，为进一步了解AEV在中国的传播提供了依据。
