PDF(Pubmed)
Abstract：
UNASSIGNED: Acute rheumatic fever (ARF) is a serious post-infectious sequala of Group A Streptococcus (GAS, Streptococcus pyogenes). In New Zealand (NZ) ARF is a major cause of health inequity. This study describes the genomic analysis of GAS isolates associated with childhood skin and throat infections in Auckland NZ.
UNASSIGNED: Isolates (n = 469) collected between March 2018 and October 2019 from the throats and skin of children (5-14 years) underwent whole genomic sequencing. Equal representation across three ethnic groups was ensured through sample quotas with isolates obtained from Indigenous Māori (n = 157, 33%), NZ European/Other (n = 149, 32%) and Pacific Peoples children (n = 163, 35%). Using in silico techniques isolates were classified, assessed for diversity, and examined for distribution differences between groups. Comparisons were also made with GAS strains identified globally.
UNASSIGNED: Genomic analysis revealed a diverse population consisting of 65 distinct sequence clusters. These sequence clusters spanned 49 emm-types, with 11 emm-types comprised of several, distinct sequence clusters. There is evidence of multiple global introductions of different lineages into the population, as well as local clonal expansion. The M1UK lineage comprised 35% of all emm1 isolates.
UNASSIGNED: The GAS population was characterized by a high diversity of strains, resembling patterns observed in low- and middle-income countries. However, strains associated with outbreaks and antimicrobial resistance commonly found in high-income countries were also observed. This unique combination poses challenges for vaccine development, disease management and control.
UNASSIGNED: The work was supported by the Health Research Council of New Zealand (HRC), award number 16/005.
UNASSIGNED: Isolates (n = 469) collected between March 2018 and October 2019 from the throats and skin of children (5-14 years) underwent whole genomic sequencing. Equal representation across three ethnic groups was ensured through sample quotas with isolates obtained from Indigenous Māori (n = 157, 33%), NZ European/Other (n = 149, 32%) and Pacific Peoples children (n = 163, 35%). Using in silico techniques isolates were classified, assessed for diversity, and examined for distribution differences between groups. Comparisons were also made with GAS strains identified globally.
UNASSIGNED: Genomic analysis revealed a diverse population consisting of 65 distinct sequence clusters. These sequence clusters spanned 49 emm-types, with 11 emm-types comprised of several, distinct sequence clusters. There is evidence of multiple global introductions of different lineages into the population, as well as local clonal expansion. The M1UK lineage comprised 35% of all emm1 isolates.
UNASSIGNED: The GAS population was characterized by a high diversity of strains, resembling patterns observed in low- and middle-income countries. However, strains associated with outbreaks and antimicrobial resistance commonly found in high-income countries were also observed. This unique combination poses challenges for vaccine development, disease management and control.
UNASSIGNED: The work was supported by the Health Research Council of New Zealand (HRC), award number 16/005.
摘要：
■急性风湿热（ARF）是A组链球菌（GAS，化脓性链球菌）。在新西兰（NZ），ARF是健康不平等的主要原因。这项研究描述了与新西兰奥克兰儿童皮肤和喉咙感染相关的GAS分离株的基因组分析。
■在2018年3月至2019年10月期间从儿童（5-14岁）的喉咙和皮肤收集的分离株（n=469）进行了全基因组测序。通过从土著毛利人获得的分离株的样本配额(n=157，33%)，确保了三个族裔群体的平等代表性，新西兰欧洲/其他（n=149，32％）和太平洋人民儿童（n=163，35％）。使用计算机技术对分离株进行分类，评估多样性，并检查了组间的分布差异。还与全球鉴定的GAS菌株进行了比较。
■基因组分析揭示了由65个不同序列簇组成的多样化群体。这些序列簇跨越49种emm类型，有11种EMM类型由几种组成，不同的序列簇。有证据表明，在全球范围内多次将不同的血统引入人口中，以及局部克隆扩张。M1UK谱系占所有emm1分离株的35%。
■GAS种群的特征是菌株的高度多样性，与低收入和中等收入国家观察到的模式相似。然而,还观察到与高收入国家常见的暴发和抗菌素耐药性相关的菌株.这种独特的组合对疫苗开发提出了挑战，疾病管理和控制。
■这项工作得到了新西兰健康研究理事会（HRC）的支持，奖励编号16/005。
■在2018年3月至2019年10月期间从儿童（5-14岁）的喉咙和皮肤收集的分离株（n=469）进行了全基因组测序。通过从土著毛利人获得的分离株的样本配额(n=157，33%)，确保了三个族裔群体的平等代表性，新西兰欧洲/其他（n=149，32％）和太平洋人民儿童（n=163，35％）。使用计算机技术对分离株进行分类，评估多样性，并检查了组间的分布差异。还与全球鉴定的GAS菌株进行了比较。
■基因组分析揭示了由65个不同序列簇组成的多样化群体。这些序列簇跨越49种emm类型，有11种EMM类型由几种组成，不同的序列簇。有证据表明，在全球范围内多次将不同的血统引入人口中，以及局部克隆扩张。M1UK谱系占所有emm1分离株的35%。
■GAS种群的特征是菌株的高度多样性，与低收入和中等收入国家观察到的模式相似。然而,还观察到与高收入国家常见的暴发和抗菌素耐药性相关的菌株.这种独特的组合对疫苗开发提出了挑战，疾病管理和控制。
■这项工作得到了新西兰健康研究理事会（HRC）的支持，奖励编号16/005。
