PDF(Pubmed)
Abstract：
Gastric cancer (GC) is one of the deadliest tumors due to its competence to invade and metastasize. The DNA repair gene (XRCC1), interleukin-8 (IL-8) gene, and B-cell lymphoma 2 (Bcl-2) gene play a crucial role in the development and progression of GC. This study aimed to evaluate the expression of these target genes in GC patients in the Kurdistan region of Iraq. Gastric cancer tissues were collected from 29 patients diagnosed with gastric adenocarcinoma that underwent gastric resection, and 21 tissue samples were obtained from healthy patients that underwent gastroscopy. The gastric tissues were collected in different hospitals in Erbil and Sulaymaniyah cities in the Kurdistan region of Iraq. Moreover, the data regarding Helicobacter pylori, age, gender, and stage of the disease were recorded and analyzed using GraphPad Prism. The gene expression levels of XRCC1, IL-8, and Bcl-2 from gastric tissue were studied by real-time quantitative polymerase chain reaction. The results showed that H. pylori infection was equally distributed among males and females in the tissues of gastric patients, while most of the H. pylori-negative patients were females. It is also found that gastric patients aged 30-60 years old are more commonly tested for the H. pylori test. Accordingly, in this study, patients diagnosed with gastric inflammation more often tested positive for H. pylori, while patients diagnosed with gastric cancer tested negative for this infection. Additionally, it was found that the target genes (XRCC1, IL-8, and Bcl-2) were significantly upregulated in GC patients, compared to the healthy group. Finally, the result revealed that XRCC1, IL-8, and Bcl-2 were upregulated in the Kurdish patients with GC, compared to the healthy control group. Targeting XRCC1, IL-8, and Bcl-2 genes can be an interesting field and promising strategy for cancer treatment.
摘要：
胃癌（GC）是最致命的肿瘤之一，由于其侵袭和转移的能力。DNA修复基因(XRCC1),白细胞介素-8（IL-8）基因，B细胞淋巴瘤2(Bcl-2)基因在GC的发生、发展中起着至关重要的作用。这项研究旨在评估这些靶基因在伊拉克库尔德斯坦地区GC患者中的表达。收集29例诊断为胃腺癌并接受胃切除术的患者的胃癌组织，和21个组织样本是从接受胃镜检查的健康患者获得的。胃组织是在伊拉克库尔德斯坦地区埃尔比勒和苏莱曼尼亚市的不同医院收集的。此外，关于幽门螺杆菌的数据，年龄,性别,使用GraphPadPrism记录和分析疾病的阶段。通过实时定量聚合酶链反应研究胃组织中XRCC1，IL-8和Bcl-2的基因表达水平。结果显示，幽门螺杆菌感染在胃癌患者的组织中男女分布均匀，而大多数幽门螺杆菌阴性患者是女性。还发现年龄在30-60岁的胃部患者更常用于幽门螺杆菌测试。因此，在这项研究中，被诊断患有胃部炎症的患者更经常幽门螺杆菌检测呈阳性，而被诊断为胃癌的患者对这种感染的检测呈阴性。此外,发现目标基因（XRCC1，IL-8和Bcl-2）在GC患者中显著上调，与健康组相比。最后,结果显示，XRCC1，IL-8和Bcl-2在患有GC的库尔德患者中上调，与健康对照组相比。靶向XRCC1，IL-8和Bcl-2基因可能是一个有趣的领域和有希望的癌症治疗策略。
