PDF(Pubmed)
Abstract：
Goats are the earliest domesticated ruminants. The local goat, Capra hircus, is considered one of the most important animals globally to provide good livestock production under harsh environmental conditions. This study aimed to detect the genetic structures of the local Iraqi goats bred in the central and southern regions of the country and investigate the possibility of benefiting from their genetic structures to construct improvement programs for increasing the productivity of these animals. To this end, blood samples were taken from 15 domestic black goats. A total of 10 ml of each animal\'s blood was placed in plastic containers of 10 ml. The DNA was extracted and sent to the laboratories of Juan Ju University, People\'s Republic of China, to analyze the sequences of the nitrogenous bases of the Cytochrome b (Cytb) gene. The results showed the presence of a genetic morphology for a segment of 670 base pairs for all the studied samples, and 15 sequences of this strain were recorded in the gene bank under the following accession numbers (LC496353.1:1-LC496367.1:1). The sequences of the nitrogenous bases of this segment of the gene, which were registered in the gene bank of some international goat breeds, were used for comparison with the sequences of black Iraqi goats to analyze the phylogenetic tree, calculate the genetic distance, study haplotypes, and calculate neutrality. The results showed the presence of one mutation in the studied segment of the Cytb gene, with a size of 670 bp. The mutation in base 46 of the studied gene converted from the purine group to the pyrimidine group (the shift from the nitrogen leaders A
摘要：
山羊是最早驯化的反刍动物。当地的山羊,CapraHircus,被认为是在恶劣的环境条件下提供良好牲畜生产的全球最重要的动物之一。这项研究旨在检测在该国中部和南部地区饲养的当地伊拉克山羊的遗传结构，并研究从其遗传结构中受益的可能性，以构建提高这些动物生产力的改进计划。为此,血液样本取自15只家养黑山羊。将总共10ml的每只动物的血液置于10ml的塑料容器中。DNA被提取并送到JuanJu大学的实验室，中华人民共和国,分析细胞色素b（Cytb）基因的含氮碱基序列。结果表明，所有研究样品的670个碱基对片段都存在遗传形态，并且该菌株的15个序列以以下登录号(LC496353.1:1-LC496367.1:1)记录在基因库中。该基因片段的含氮碱基序列，在一些国际山羊品种的基因库中注册，用于与伊拉克黑山羊的序列进行比较，以分析系统发育树，计算遗传距离，研究单倍型，计算中立。结果表明，在Cytb基因的研究片段中存在一个突变，大小为670bp。在所有研究的样品中,所研究基因的碱基46的突变从嘌呤组转变为嘧啶组(从氮前导序列A
