PDF(Pubmed)
Abstract：
The glycolytic pathway involves phosphofructokinase (PFK), a rate-limiting enzyme that catalyzes the phosphorylation of fructose-6-phosphate. In plants, the two PFK members are ATP-dependent phosphofructokinase (PFK) and pyrophosphate-fructose-6-phosphate phosphotransferase (PFP). However, the functions of the PFK family members in Quercus rubra are not well understood. The purpose of this study was to investigate the genome-wide distribution of the PFK family members and their roles in Q. rubra by performing a systematic study of the phylogenetic relationships, molecular characteristics, motifs, chromosomal and subcellular locations, and cis-elements of QrPFKs. We identified 14 QrPFK genes in the genome of Q. rubra, followed by examining their expression in different tissues, including the roots, stems, and leaves. The phylogenetic tree divided the 14 QrPFK genes into two groups: 11 belonging to PFK and three belonging to PFP. The expression profiles of all 14 proteins were relatively the same in leaves but differed between stems and roots. Four genes (Qurub.02G189400.1, Qurub.02G189400.2, Qurub.09G134300.1, and Qurub.09G134300.2) were expressed at very low levels in both stems and roots, while two (Qurub.05G235500.1 and Qurub.05G235500.1) were expressed at low levels and the others showed relatively high expression in all tissues.
摘要：
糖酵解途径涉及磷酸果糖激酶（PFK），一种催化果糖-6-磷酸磷酸化的限速酶。在植物中,两个PFK成员是ATP依赖性磷酸果糖激酶（PFK）和焦磷酸-果糖-6-磷酸磷酸转移酶（PFP）。然而,PFK家族成员的功能尚不清楚。这项研究的目的是通过对系统发育关系进行系统研究来调查PFK家族成员的全基因组分布及其在Q.rubra中的作用，分子特征，图案，染色体和亚细胞位置，和QrPFK的顺式元素。我们在Q.rubra的基因组中鉴定了14个QrPFK基因，然后检查它们在不同组织中的表达，包括根，茎,和树叶。系统发育树将14个QrPFK基因分为两组：11个属于PFK，3个属于PFP。所有14种蛋白质的表达谱在叶片中相对相同，但在茎和根之间有所不同。四个基因（Qurub.02G189400.1，Qurub.02G189400.2，Qurub.09G134300.1和Qurub.09G134300.2）在茎和根中都以非常低的水平表达，而两个（Qurub.05G235500.1和Qurub.05G235500.1）在所有组织中的表达水平较低，其他表达水平较高。
