PDF(Pubmed)
Abstract：
Canola (Brassica napus L.) meal represents a prominent alternative plant-based source for protein isolation. This work aimed to investigate the combined effect of extraction and purification methods for the production of canola protein isolates (CPIs). CPIs were characterized in terms of process yield, protein recovery, basic composition, amino acid profile, in vitro protein digestibility, techno-functional properties, structural properties, and molecular features. The results showed that the Alk-Uf method enhanced yield (16.23 %) and protein recovery (34.88 %). Meanwhile, the Et-Alk-Uf method exhibited the highest crude protein (89.71 %) and free amino nitrogen (4.34 mg g protein-1) contents. Furthermore, protein digestibility (95.5 %) and protein digestibility corrected amino acid score (1.0) were improved using the Et-Alk-Ac method. Conversely, the amino acid composition, secondary structure, and electrophoretic profiles were generally similar for all CPIs. The Alk-Uf and Et-Alk-Uf methods produced isolates with the highest water solubility (∼39.18 %), water absorption capacity (∼3.86 g water g protein-1), oil absorption capacity (∼2.77 g oil g protein-1), and foaming capacity (∼505.26 %). Finally, the foaming stability (93.75 %) and foaming density (34.38 %) were increased when employing the Alk-Ac method. These findings suggest that, in general, the Alk-Uf and Et-Alk-Uf methods can be used to obtain CPIs with high added value for use in food formulations.
摘要：
油菜（BrassicanapusL.）粉代表了一种用于蛋白质分离的突出的替代植物来源。本工作旨在研究提取和纯化方法对油菜分离蛋白（CPIs）生产的联合作用。CPIs的特征在于工艺产量，蛋白质回收，基本组成，氨基酸谱,体外蛋白质消化率，技术功能特性，结构特性，和分子特征。结果表明，Alk-Uf法提高了收率（16.23％）和蛋白质回收率（34.88％）。同时,Et-Alk-Uf法表现出最高的粗蛋白（89.71％）和游离氨基氮（4.34mggprotein-1）含量。此外，使用Et-Alk-Ac方法，蛋白质消化率（95.5％）和蛋白质消化率校正的氨基酸评分（1.0）得到改善。相反，氨基酸组成,二级结构,所有CPIs的电泳图谱通常相似。Alk-Uf和Et-Alk-Uf方法产生的分离物具有最高的水溶性（〜39.18％），吸水能力(~3.86g水g蛋白-1)，吸油能力(~2.77g油g蛋白-1)，和发泡能力（~505.26%）。最后,当采用Alk-Ac法时，发泡稳定性（93.75％）和发泡密度（34.38％）增加。这些发现表明,总的来说,Alk-Uf和Et-Alk-Uf方法可用于获得用于食品配方的具有高附加值的CPIs。
