PDF(Pubmed)
Abstract：
UNASSIGNED: Since the first human case of Middle East Respiratory Syndrome (MERS) caused by Coronavirus (MERS-CoV) in 2012, several evidence bases have shown one-humped camels as the main reservoir host, from which infection is transmitted to humans.
UNASSIGNED: Serological investigation of MERS in dromedary camels in Wasit province (Iraq), detection severity of infection, and association to some risk factors.
UNASSIGNED: A total of 455 dromedary camels were selected randomly from two main districts in Wasit province, Iraq, during January and April (2023). Sera of all study camels were examined by enzyme-linked immunosorbent assay (ELISA), and titers of positive study animals were categorized according to their severity.
UNASSIGNED: Serological testing yielded 37.58% positive animals for MERS infection. According to the severity of positive ODs (titer), a total of 53.22%, 30.99%, 12.28%, and 3.51% showed mild, moderate, strong, and very strong infections, respectively. Regarding risk factors, significant elevation in seropositivity was seen in camels of >3-6 and >6 years old and reduced in camels of £3 years old with an elevated risk of MERS with increased age. Regionally, seropositivity and relative risk were increased in the camels of Shaykh Sa\'d when compared with Al-Numaniyah. Regarding sex, no significant variation was detected between seropositive females and males; however, male camels appeared at higher risk than females. Association between the severity of MERS infection and risk factors revealed that there was a significant increase in mild and moderate infections in female camels of >6 years old; whereas strong and very strong infections were seen in male camels of 33-6 years old. Mild and very strong infections were recorded in Shaykh Sa\'d; while moderate and strong infections in Al-Numaniyah.
UNASSIGNED: The study indicated a longstanding existence of MERS-CoV in camels of Wasit province; therefore, recent infections or active viral excretion are required for confirmation by molecular approaches.
UNASSIGNED: Serological investigation of MERS in dromedary camels in Wasit province (Iraq), detection severity of infection, and association to some risk factors.
UNASSIGNED: A total of 455 dromedary camels were selected randomly from two main districts in Wasit province, Iraq, during January and April (2023). Sera of all study camels were examined by enzyme-linked immunosorbent assay (ELISA), and titers of positive study animals were categorized according to their severity.
UNASSIGNED: Serological testing yielded 37.58% positive animals for MERS infection. According to the severity of positive ODs (titer), a total of 53.22%, 30.99%, 12.28%, and 3.51% showed mild, moderate, strong, and very strong infections, respectively. Regarding risk factors, significant elevation in seropositivity was seen in camels of >3-6 and >6 years old and reduced in camels of £3 years old with an elevated risk of MERS with increased age. Regionally, seropositivity and relative risk were increased in the camels of Shaykh Sa\'d when compared with Al-Numaniyah. Regarding sex, no significant variation was detected between seropositive females and males; however, male camels appeared at higher risk than females. Association between the severity of MERS infection and risk factors revealed that there was a significant increase in mild and moderate infections in female camels of >6 years old; whereas strong and very strong infections were seen in male camels of 33-6 years old. Mild and very strong infections were recorded in Shaykh Sa\'d; while moderate and strong infections in Al-Numaniyah.
UNASSIGNED: The study indicated a longstanding existence of MERS-CoV in camels of Wasit province; therefore, recent infections or active viral excretion are required for confirmation by molecular approaches.
摘要：
■自2012年第一例由冠状病毒（MERS-CoV）引起的中东呼吸综合症（MERS）人类病例以来，一些证据基础表明，单峰骆驼是主要的水库宿主，从感染传播给人类。
■在瓦西特省（伊拉克）单峰骆驼中对MERS的血清学调查，检测感染的严重程度，以及与一些风险因素的关联。
■从瓦西特省的两个主要地区随机选择了455头单峰骆驼，伊拉克,1月和4月（2023年）。通过酶联免疫吸附试验（ELISA）检查所有研究骆驼的血清，阳性研究动物的滴度根据其严重程度进行分类。
■血清学测试产生37.58%的MERS感染阳性动物。根据ODs阳性（滴度）的严重程度，合计为53.22%,30.99%,12.28%，3.51%表现温和，中度,坚强,和非常强烈的感染，分别。关于风险因素，血清阳性在>3-6岁和>6岁的骆驼中显著升高,在£3岁的骆驼中降低,随着年龄的增加,MERS的风险升高.区域,与Al-Numaniyah相比，ShaykhSa'd骆驼的血清阳性和相对风险增加。关于性,在血清阳性的女性和男性之间没有检测到显著的变异；然而，雄性骆驼的风险高于雌性。MERS感染的严重程度与危险因素之间的关联表明，>6岁的雌性骆驼的轻度和中度感染显着增加；而在33-6岁的雄性骆驼中发现了强烈和非常强烈的感染。在ShaykhSa\'d中记录了轻度和非常强烈的感染；而Al-Numaniyah中的中度和强烈感染。
■该研究表明，在瓦西特省的骆驼中长期存在MERS-CoV；因此，最近的感染或活跃的病毒排泄需要通过分子方法进行确认。
■在瓦西特省（伊拉克）单峰骆驼中对MERS的血清学调查，检测感染的严重程度，以及与一些风险因素的关联。
■从瓦西特省的两个主要地区随机选择了455头单峰骆驼，伊拉克,1月和4月（2023年）。通过酶联免疫吸附试验（ELISA）检查所有研究骆驼的血清，阳性研究动物的滴度根据其严重程度进行分类。
■血清学测试产生37.58%的MERS感染阳性动物。根据ODs阳性（滴度）的严重程度，合计为53.22%,30.99%,12.28%，3.51%表现温和，中度,坚强,和非常强烈的感染，分别。关于风险因素，血清阳性在>3-6岁和>6岁的骆驼中显著升高,在£3岁的骆驼中降低,随着年龄的增加,MERS的风险升高.区域,与Al-Numaniyah相比，ShaykhSa'd骆驼的血清阳性和相对风险增加。关于性,在血清阳性的女性和男性之间没有检测到显著的变异；然而，雄性骆驼的风险高于雌性。MERS感染的严重程度与危险因素之间的关联表明，>6岁的雌性骆驼的轻度和中度感染显着增加；而在33-6岁的雄性骆驼中发现了强烈和非常强烈的感染。在ShaykhSa\'d中记录了轻度和非常强烈的感染；而Al-Numaniyah中的中度和强烈感染。
■该研究表明，在瓦西特省的骆驼中长期存在MERS-CoV；因此，最近的感染或活跃的病毒排泄需要通过分子方法进行确认。
