PDF(Pubmed)
Abstract：
The aim of the present study was to evaluate semen cryopreservation with ACP-Lact® diluent, which consists of coconut water powder (ACP) added to goat milk powder. After thawing, the samples were evaluated for sperm kinetics, membrane evaluation and in vivo insemination. For cryopreservation, a pool was made with the ejaculate of six goats, diluted in four equal aliquots for the respective treatments: T1 (ACP-Lact®); T2 (ACP-Lact® 50%); T3 (ACP + 2.5% egg yolk) and T4 (Tris + 2.5% egg yolk). After dilution of the treatments, the samples were placed in 0.5 ml straws and chilled at a rate of -1.07°C/min. After reaching 4°C and stabilizing for one hour, the straws were placed in nitrogen vapour at -60°C for 15 minutes and then immersed in liquid nitrogen (-196ºC). The straws were thawed in a 37°C water bath and kinetic assessments were performed immediately using a computerized semen analysis program (CSA), viability (EN), membrane functionality (HOST), mitochondrial activity (DAB) and DNA integrity assessment of spermatozoa. For the in vivo experiment, ten goats were inseminated, divided into two groups of five goats each, G1 inseminated with ACP-Lact® and G2 with ACP, by fixed-time artificial insemination (FTAI). Regarding the kinetic parameters, the ACP-Lact® treatment showed higher progressive motility (PM) and sperm velocity than the other treatments (36.77%). In the VSL parameter the ACP-Lact diluent was superior to ACP and Tris. In viability the treatment with ACP-Lact® was superior to the treatment with Tris, 95% and 83% respectively. In FTAI two goats were born out of the 5 goats inseminated with ACP-Lact®. It was concluded that the use of ACP-Lact® for cryopreservation of caprine semen is efficient in maintaining seminal parameters during thawing in vitro and in vivo and proved to be a good alternative extender for the caprine species.
摘要：
本研究的目的是评估ACP-Lact®稀释剂的精液冷冻保存，由添加到羊奶粉中的椰子水粉（ACP）组成。解冻后,对样本进行精子动力学评估，膜评价和体内授精。对于冷冻保存，用六只山羊的射精建造了一个游泳池，在四个相等的等分试样中稀释,用于各自的处理:T1(ACP-Lact®);T2(ACP-Lact®50%);T3(ACP+2.5%蛋黄)和T4(Tris+2.5%蛋黄)。稀释处理后，将样品置于0.5ml吸管中并以-1.07°C/min的速率冷却。达到4°C并稳定一小时后，将吸管置于-60°C的氮气蒸汽中15分钟，然后浸入液氮（-196ºC）中。将吸管在37°C水浴中解冻，并立即使用计算机精液分析程序（CSA）进行动力学评估。生存能力（EN），膜功能（主机），精子的线粒体活性（DAB）和DNA完整性评估。对于体内实验，十只山羊被授精，分成两组，每组五只山羊，G1用ACP-Lact®授精，G2用ACP授精，通过固定时间人工授精（FTAI）。关于动力学参数，与其他治疗相比,ACP-Lact®治疗显示更高的进行性运动(PM)和精子速度(36.77%).在VSL参数中,ACP-Lact稀释剂优于ACP和Tris。在生存力方面，ACP-Lact®治疗优于Tris治疗，分别为95%和83%。在FTAI中，用ACP-Lact®授精的5只山羊中有两只山羊出生。结论是，使用ACP-Lact®冷冻保存山羊精液在体外和体内解冻过程中可有效维持精液参数，并被证明是山羊物种的良好替代品。
