PDF(Pubmed)
Abstract：
Introduction Forensic science has continually evolved, with innovations in DNA analysis techniques playing a pivotal role in improving the accuracy and reliability of criminal investigations. Short tandem repeats (STRs) have been a game-changer in forensic science, offering unique genetic markers to pinpoint individual identities. This study explores the application of STR DNA analysis to an unconventional source: coffee cups. Materials and methods In a study involving 16 unrelated, healthy individuals aged 26 to 32, DNA was investigated from coffee cups and mouth cavities, comparing the results to a previous coffee cup DNA study. Buccal swabs provided reference samples, air-dried for 10 minutes, and stored at 20°C. DNA quantification revealed a significant decrease in coffee cup samples (0.015 to 0.049 ng/μL) compared to the reference, with no DNA in negative controls. Some STR loci displayed inhibition and lower heterozygosity in the research samples. Ethical approval (REC 53-592) and adherence to the Declaration of Helsinki ensured ethical standards. This research highlights potential challenges in obtaining efficient DNA profiles from various sources. Results There was a significant variation in DNA concentrations among the different cup types, with ceramic cups yielding the highest concentrations. Moreover, the DNA profiling outcomes ranged from full profiles, which allow for precise individual identification, to partial profiles, which pose challenges for conclusive matches. These findings have profound implications for forensic science. The study demonstrates the potential of coffee cups as sources of DNA evidence in criminal investigations, even if partial profiles are obtained. Such evidence can assist in suspect identification, victim profiling, and corroborating witness statements. Conclusion This study highlights the application of STR DNA analysis for coffee cups, opening new avenues for forensic investigations and highlighting the need for continued research and development in this exciting field. This observation aligns with existing research on DNA recovery from various surfaces, and further research is warranted to refine the methodology, consider usage patterns, and address ethical and legal concerns regarding the collection and use of DNA evidence from common objects.
摘要：
介绍法医科学不断发展，DNA分析技术的创新在提高刑事调查的准确性和可靠性方面发挥着关键作用。短串联重复(STR)一直是法医学的游戏规则改变者，提供独特的遗传标记来确定个人身份。这项研究探讨了STRDNA分析在非常规来源：咖啡杯中的应用。材料和方法在一项涉及16项无关的研究中，26至32岁的健康个体，从咖啡杯和口腔中调查DNA，将结果与之前的咖啡杯DNA研究进行比较。颊拭子提供了参考样本，风干10分钟,并储存在20℃。DNA定量显示，与参考相比，咖啡杯样品（0.015至0.049ng/μL）显着减少，阴性对照中没有DNA.一些STR基因座在研究样品中表现出抑制和较低的杂合性。伦理批准（REC53-592）和遵守赫尔辛基宣言确保了道德标准。这项研究强调了从各种来源获得有效DNA图谱的潜在挑战。结果不同杯型之间DNA浓度存在显著差异,陶瓷杯产生最高的浓度。此外，DNA分析结果从完整的概况，允许精确的个人识别，到部分剖面，这对决定性的比赛构成了挑战。这些发现对法医学具有深远的意义。这项研究证明了咖啡杯在刑事调查中作为DNA证据来源的潜力，即使获得了部分轮廓。这些证据可以帮助识别嫌疑人，受害者分析,并证实证人的证词.结论本研究突出了STRDNA分析在咖啡杯中的应用,为法医调查开辟了新的途径，并强调了在这个令人兴奋的领域继续研究和发展的必要性。这一观察结果与现有的从各种表面回收DNA的研究一致，需要进一步的研究来完善方法论，考虑使用模式，并解决有关从常见物体中收集和使用DNA证据的道德和法律问题。
