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Abstract：
The rete testis (RT) is a region of the mammalian testis that plays an important role in testicular physiology. The RT epithelium consists of cells sharing some well-known gene markers with supporting Sertoli cells (SCs). However, little is known about the differences in gene expression between these two cell populations. Here, we used fluorescence-activated cell sorting (FACS) to obtain pure cultures of neonatal RT cells and SCs and identified differentially expressed genes (DEGs) between these cell types using RNA sequencing (RNA-seq). We then compared our data with the RNA-seq data of other studies that examined RT cells and SCs of mice of different ages and generated a list of DEGs permanently upregulated in RT cells throughout testis development and in culture, which included 86 genes, and a list of 79 DEGs permanently upregulated in SCs. The analysis of studies on DMRT1 function revealed that nearly half of the permanent DEGs could be regulated by this SC upregulated transcription factor. We suggest that useful cell lineage markers and candidate genes for the specification of both RT cells and SCs may be present among these permanent DEGs.
摘要：
睾丸网（RT）是哺乳动物睾丸的一个区域，在睾丸生理学中起着重要作用。RT上皮由与支持支持支持支持支持细胞（SC）共享一些众所周知的基因标记的细胞组成。然而,关于这两个细胞群体之间的基因表达差异知之甚少。这里,我们使用荧光激活细胞分选术(FACS)获得新生儿RT细胞和SCs的纯培养物,并使用RNA测序(RNA-seq)鉴定了这些细胞类型之间的差异表达基因(DEGs).然后，我们将我们的数据与其他研究的RNA-seq数据进行了比较，这些研究检查了不同年龄小鼠的RT细胞和SC，并生成了一系列在整个睾丸发育和培养过程中在RT细胞中永久上调的DEG。其中包括86个基因，以及在SC中永久上调的79个DEGs列表。对DMRT1功能的研究分析表明，该SC上调的转录因子可以调节将近一半的永久性DEG。我们建议在这些永久性DEG中可能存在有用的细胞谱系标记和用于确定RT细胞和SC的候选基因。
