PDF(Pubmed)
Abstract：
Immune disorders caused by sepsis have recently drawn much attention. We sought to dynamically monitor the expression of small extracellular vesicle (sEV) miRNAs in peripheral blood during sepsis to explore these miRNAs as potential biomarkers for monitoring immune function in sepsis patients. This study included patients with sepsis. Blood samples were obtained from 10 patients on the first through 10th days, the 12th day and the 14th day since sepsis onset, resulting in 120 collected samples. Serum sEVs were extracted from peripheral venous blood, and levels of MIR497HG, miR-195, miR-497, and PD-L1 in serum sEVs were detected by qPCR, and clinical information was recorded. Our study revealed that the levels of MIR497HG, miR-195, miR-497 and PD-L1 in serum sEVs showed periodic changes; the time from peak to trough was approximately 4-5 days. The levels of sEV MIR497HG and miR-195 had a positive linear relationship with SOFA score (r values were -0.181 and -0.189; p values were 0.048 and 0.039, respectively). The recorded quantities of sEV MIR497HG, miR-195 and PD-L1 showed a substantial correlation with ARDS. ROC curve analysis revealed that sEV MIR497HG, miR-195 and miR-497 could predict the 28-day mortality of sepsis patients with an AUC of 0.66, 0.68 and 0.72, respectively. Levels of sEVs MIR497HG, miR-195, miR-497 and PD-L1 showed periodic changes with the immune status of sepsis, which provides a new exploration direction for immune function biomarkers and immunotherapy timing in sepsis patients.
摘要：
脓毒症引起的免疫紊乱最近引起了很多关注。我们试图动态监测脓毒症患者外周血中小细胞外囊泡（sEV）miRNA的表达，以探索这些miRNA作为监测脓毒症患者免疫功能的潜在生物标志物。这项研究包括脓毒症患者。在第1天至第10天从10名患者中获得血液样本，自败血症发作以来的第12天和第14天，收集了120个样本。从外周静脉血中提取血清sEV，和MIR497HG的水平，qPCR检测血清sEV中miR-195、miR-497和PD-L1,并记录临床信息。我们的研究表明，MIR497HG的水平，血清sEV中miR-195、miR-497和PD-L1呈周期性变化；从高峰到低谷的时间约为4-5天。sEVMIR497HG和miR-195水平与SOFA评分呈正线性关系（r值分别为-0.181和-0.189；p值分别为0.048和0.039）。记录的sEVMIR497HG数量，miR-195和PD-L1与ARDS有显著相关性。ROC曲线分析显示，sEVMIR497HG，miR-195和miR-497可以预测脓毒症患者的28天死亡率，AUC分别为0.66、0.68和0.72。sEVMIR497HG的水平，miR-195、miR-497和PD-L1随脓毒症免疫状态呈周期性变化,为脓毒症患者的免疫功能生物标志物和免疫治疗时机提供了新的探索方向。
