Abstract：
The role of tetraspanin CD81 in malignant transformation is best studied in colorectal cancer, and it appears that other transcripts beside the fully coding mRNA may also be dysregulated in malignant cells. Recent data from a comprehensive pan-cancer transcriptome analysis demonstrated differential activity of two alternative CD81 gene promoters in malignant versus nonmalignant gut mucosa. The promoter active in gut mucosa gives rise to transcripts CD81-203 and CD81-213, while the promoter active in colon and rectal cancer gives rise to transcripts CD81-205 and CD81-215. Our study aimed to explore the biomarker potential of the transcripts from the alternative CD81 gene promoters in colon cancer, as well as to investigate their structure and potential function using in silico tools. The analysis of the transcripts\' expression in several colon cell lines cultivated in 2D and 3D and a set of colon cancer and healthy gut mucosa samples by qPCR and RNA sequencing suggested their low expression and stromal origin. Expression patterns in tumor and nontumor tissue along with in silico data suppose that the transcript CD81-215 may be a noncoding RNA of stromal origin with possible involvement in signaling related to malignant transformation.
摘要：
四跨膜蛋白CD81在恶性转化中的作用在结直肠癌中得到了最好的研究，似乎除了完全编码的mRNA之外，其他转录本也可能在恶性细胞中失调。来自全面的泛癌症转录组分析的最新数据表明，两种替代的CD81基因启动子在恶性和非恶性肠粘膜中的差异活性。在肠粘膜中有活性的启动子产生转录物CD81-203和CD81-213,而在结肠癌和直肠癌中有活性的启动子产生转录物CD81-205和CD81-215。我们的研究旨在探索结肠癌中替代CD81基因启动子转录本的生物标志物潜力，以及使用硅片工具研究它们的结构和潜在功能。通过qPCR和RNA测序对在2D和3D中培养的几种结肠细胞系以及一组结肠癌和健康肠粘膜样品中的转录物\'表达的分析表明它们的低表达和基质起源。肿瘤和非肿瘤组织中的表达模式以及计算机模拟数据假设转录本CD81-215可能是基质来源的非编码RNA，可能参与与恶性转化有关的信号传导。
