PDF(Pubmed)
Abstract：
Helminth infections are a worldwide problem that affects both humans and animals in developing countries. The common pinworm Syphacia muris frequently infects lab rats and can obstruct the creation of unrelated biological experiments. The objective of this study was to examine the in vivo efficacy of silver nanoparticles against S. muris infected Wistar rats. Transmission electron microscopy and X-ray diffraction examinations of silver nanoparticles revealed highly pure polycrystals with a mean size of 4 nm. Rats were divided into group I, the control: received distilled water; groups II and III, the treated: received 2, 4 mg/kg b.w. of Ag NPs, respectively. At the end of the experimental period, all rats were euthanized and dissected for collecting worms. The surface topography of the recovered worms was displayed using light and scanning electron microscopy, and their physiological status was determined using oxidative stress biomarkers. The histological changes in the rat liver, kidney, and spleen were also examined. In the current study, Ag NPs administration revealed substantial alterations in worms collected from treated rats, including shrinkage of lips, peeling and rupture of body cuticles, and disruption of surface annulations. Also, induced a significant increase in malondialdehyde and nitric oxide levels, as well as a decrease in reduced glutathione, glutathione peroxidase and catalase levels compared to control group. Moreover, sections of treated rats\' liver, kidney and spleen displayed normal cellular appearance. In conclusion, this is the first in vivo study to evaluate Ag NPs efficacy against S. muris in laboratory rats without significant toxicity.
摘要：
蠕虫感染是一个世界性问题，影响发展中国家的人类和动物。常见的pin虫Syphaciamuris经常感染实验室大鼠，并可能阻碍无关生物学实验的创建。这项研究的目的是检查银纳米颗粒对S.muris感染的Wistar大鼠的体内功效。银纳米颗粒的透射电子显微镜和X射线衍射检查显示出平均尺寸为4nm的高纯度多晶。将大鼠分为I组,对照组：接受蒸馏水；第II组和第III组，治疗者：接受2,4mg/kgb.w.AgNP，分别。在实验期结束时,对所有大鼠实施安乐死并解剖以收集蠕虫。使用光学和扫描电子显微镜显示了回收的蠕虫的表面形貌，并使用氧化应激生物标志物确定其生理状态。大鼠肝脏的组织学变化,肾,还检查了脾脏。在目前的研究中,AgNPs给药显示从治疗大鼠收集的蠕虫发生了实质性变化，包括嘴唇的收缩，身体角质层的剥离和破裂，和表面环状的破坏。此外，诱导丙二醛和一氧化氮水平显着增加，以及还原型谷胱甘肽的减少，谷胱甘肽过氧化物酶和过氧化氢酶水平与对照组相比。此外，治疗大鼠肝脏切片，肾脏和脾脏显示正常的细胞外观。总之,这是首次在没有明显毒性的实验大鼠体内评价AgNPs对S.muris疗效的体内研究。
