Abstract：
Sistan Yaghooti grape variety, despite characteristics such as early ripening, is vulnerable to cluster rot due to small berries and dense clusters. In this regard, AS may serve as a regulatory mechanism during developmental processes and in response to environmental signals. RNA-Seq analysis was performed to measure gene expression and the extent of AS events in the cluster growth and development stages of Sistan Yaghooti grape. The number of AS events increased during stages, suggesting that it contributes to the grapevine\'s adaptability to various stresses. In addition, DEG and DAS genes showed little overlap in cluster growth stages. Functional analysis of 19,194 DAS -gene sets showed that VIT_06s0004g06670 gene is involved in the activation of calcium channels (Ca2+) through the activation of 5 PLC biosynthetic pathways. Among the 27,229 DEG -sets, VIT_07s0005g05320 gene showed higher expression. Interestingly, this gene is involved in the synthesis of an EF -hand domain-containing protein capable of binding to Ca2+ by activating 4 biochemical pathways. These genes increase cytosolic Ca2+ concentration, enhancing plant stress tolerance and resistance to cracking. These results show that AS can respond independently to different types of stress. Among the other DAS genes, the GA2ox gene (VvGA2ox) showed an increase in AS events during cluster development. This gene is critical for initiating the degradation process of GA and plays a crucial role in different stages of seed development. Therefore, it is very likely that this gene is one of the main factors responsible for the density and seedlessness of Sistan Yaghooti grape.
摘要：
SistanYaghooti葡萄品种，尽管有早熟等特点，由于小浆果和密集的集群，容易发生集群腐烂。在这方面,AS可以在发育过程中和响应环境信号的调节机制。进行RNA-Seq分析以测量SistanYaghooti葡萄簇生长和发育阶段的基因表达和AS事件的程度。阶段期间AS事件的数量增加，这表明它有助于葡萄藤对各种压力的适应性。此外,DEG和DAS基因在簇生长阶段几乎没有重叠。对19194个DAS-基因集的功能分析表明，VIT_06s0004g06670基因通过激活5个PLC生物合成途径参与钙通道(Ca2+)的激活。在27229DEG-套装中，VIT_07s0005g05320基因显示较高表达。有趣的是,该基因参与合成含EF手结构域的蛋白质，该蛋白质能够通过激活4种生化途径与Ca2结合。这些基因增加细胞溶质Ca2+浓度，提高植物的抗逆性和抗裂能力。这些结果表明，AS可以独立地响应不同类型的压力。在其他DAS基因中，GA2ox基因（VvGA2ox）在集群发育过程中显示AS事件增加。该基因对于启动GA的降解过程至关重要，并且在种子发育的不同阶段中起着至关重要的作用。因此,该基因很可能是导致SistanYaghooti葡萄密度和无核的主要因素之一。
