PDF(Pubmed)
Abstract：
This study aimed to determine phosphorus and vitamin B12 supplementation effect in semen extender on the quality and fertility ability of chilled Thai native rooster semen. Eighty-four ejaculates of semen from 26 Thai native roosters (Burmese × Vietnam crossbreed) were included. Semen was collected by applying dorsal-abdominal massage once a week, pooled, diluted to 500 million sperms per dose, and divided into 6 groups. The semen samples used for control group were diluted with modified Beltsville poultry semen extender (BPSE). For the treatment groups 2 to 6: semen samples were diluted with modified BPSE and enriched with phosphorus and vitamin B12 (Octafos Octa Memorial Co., Ltd., Bangkok, Thailand) at concentrations 0.02, 0.04, 0.06, 0.08, and 0.10%. Semen fertility ability was tested in 6 replications by inseminating layer hens. Thirty-six Thai native hens were randomly assigned to 3 groups (control, 0.04, and 0.08%) of 12 hens and were inseminated with a dose of 0.2 mL on collecting day. Sperm motion characteristics (i.e., sperm motility, sperm progressive motility, and sperm kinetic parameters) were measured using a computer-assisted sperm analysis system (SCA, Proiser S.L., Valencia, Spain). Sperm viability, mitochondrial activity, acrosome integrity, plasma membrane integrity, and malondialdehyde (MDA) concentration were also evaluated. The sperm motion characteristics were the highest in the 0.04% supplementation group on all days of collection, especially the VCL and VAP (P < 0.05). The viability, mitochondrial activity, plasma membrane and acrosome integrity of spermatozoa were greater in the 0.04% supplementation group than in the control groups (P < 0.05). The 0.04% supplementation group had the lowest MDA concentration in all days of collection. The 0.04% supplementation group were higher both fertility (66.59 vs. 48.50%: P < 0.05) and hatching rates (58.80 vs. 43.18%: P < 0.05) than in the control group. In conclusion, 0.04% phosphorus and vitamin B12 concentrations supplementation in semen extender improved rooster semen quality and fertility in chilled rooster semen.
摘要：
本研究旨在确定补充精液中磷和维生素B12对冷冻泰国本地公鸡精液质量和生育能力的影响。包括来自26只泰国本地公鸡（缅甸×越南杂交）的84种精液。每周一次应用背腹部按摩收集精液，池化,稀释到每剂5亿个精子，分为6组。用于对照组的精液样品用改良的Beltsville家禽精液补充剂（BPSE）稀释。对于治疗组2至6：用改良的BPSE稀释精液样品，并富含磷和维生素B12（OctafosOctaMemorialCo.，Ltd.,曼谷,泰国）浓度为0.02、0.04、0.06、0.08和0.10％。通过授精层母鸡在6次重复中测试了精液的生育能力。36只泰国本土母鸡被随机分为3组(对照组，0.04和0.08％）的12只母鸡，并在收集日以0.2mL的剂量进行授精。精子运动特征(即，精子运动性,精子进行性运动性，和精子动力学参数）使用计算机辅助精子分析系统（SCA，ProiserS.L.,瓦伦西亚,西班牙)。精子活力,线粒体活性，顶体完整性,质膜完整性，还评估了丙二醛（MDA）浓度。在所有收集日的0.04%补充组中，精子运动特征最高，尤其是VCL和VAP(P<0.05)。生存能力,线粒体活性，0.04%补充组精子质膜和顶体完整性高于对照组(P<0.05)。0.04%的补充组在收集的所有天数中具有最低的MDA浓度。0.04%的补充组生育率均较高(66.59vs.48.50%：P<0.05）和孵化率（58.80vs.43.18%:P<0.05)高于对照组。总之,精液补充剂中添加0.04％的磷和维生素B12浓度可改善冷藏公鸡精液的质量和繁殖力。
