PDF(Pubmed)
Abstract：
Bovine respiratory disease (BRD) is a global prevalent multifactorial infection primarily caused by viral and bacterial coinfections. In China, Mycoplasma bovis (M. bovis) and bovine herpesvirus type 1 (BoHV-1) are the predominant pathogens associated with BRD. Our previous study involved the development of attenuated M. bovis HB150 and BoHV-1 gG-/tk- vaccine strains, which were thoroughly assessed for their safety profiles and protective efficacy in cattle. In this study, we applied a combination of vaccines in varying ratios and used a rabbit model to determine the safety and protective efficacy. We used PCR/RT-PCR to detect the postimmunization and challenge shedding of M. bovis and BoHV-1. Additionally, we measured antibody titers and the expression of IFN-β and TNF-α to evaluate the humoral and cellular immune responses, respectively. Furthermore, we performed a histopathological analysis to assess lung damage. Our study provides evidence of the safety and effectiveness of the bivalent M. bovis-BoHV-1 vaccine in rabbits, particularly when applying a combination of 1.0 × 108 CFU of M. bovis HB150 and 1.0 × 106 TCID50 of the BoHV-1 gG-/tk- strain. The bivalent vaccine significantly enhanced both the long-term antibody immune response and cellular protection against the M. bovis and BoHV-1 challenge. These findings provide a valuable model for the potential application in cattle.
摘要：
牛呼吸道疾病（BRD）是主要由病毒和细菌共同感染引起的全球流行的多因素感染。在中国,牛支原体（M.牛）和牛疱疹病毒1型（BoHV-1）是与BRD相关的主要病原体。我们先前的研究涉及减毒牛分枝杆菌HB150和BoHV-1gG-/tk-疫苗株的开发，对其在牛中的安全性和保护功效进行了全面评估。在这项研究中,我们使用不同比例的疫苗组合,并使用兔模型来确定安全性和保护效果.我们使用PCR/RT-PCR检测牛分枝杆菌和BoHV-1的免疫后和攻击脱落。此外,我们测量抗体滴度和IFN-β和TNF-α的表达，以评估体液和细胞免疫应答，分别。此外，我们进行了组织病理学分析以评估肺损伤.我们的研究提供了二价牛分枝杆菌BoHV-1疫苗在兔中的安全性和有效性的证据，特别是当应用1.0×108CFU的牛分枝杆菌HB150和1.0×106TCID50的BoHV-1gG-/tk-菌株的组合时。二价疫苗显著增强了长期抗体免疫应答和针对牛分枝杆菌和BoHV-1攻击的细胞保护。这些发现为牛的潜在应用提供了有价值的模型。
