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Abstract：
Mycoplasma synoviae infection rates in chickens are increasing worldwide. Genomic studies have considerably improved our understanding of M. synoviae biology and virulence. However, approximately 20% of the predicted proteins have unknown functions. In particular, the M. synoviae ATCC 25204 genome has 663 encoding DNA sequences, among which 155 are considered encoding hypothetical proteins (HPs). Several of these genes may encode unknown virulence factors. This study aims to reannotate all 155 proteins in M. synoviae ATCC 25204 to predict new potential virulence factors using currently available databases and bioinformatics tools. Finally, 125 proteins were reannotated, including enzymes (39%), lipoproteins (10%), DNA-binding proteins (6%), phase-variable hemagglutinin (19%), and other protein types (26%). Among 155 proteins, 28 proteins associated with virulence were detected, five of which were reannotated. Furthermore, HP expression was compared before and after the M. synoviae infection of cells to identify potential virulence-related proteins. The expression of 14 HP genes was upregulated, including that of five virulence-related genes. Our study improved the functional annotation of M. synoviae ATCC 25204 from 76% to 95% and enabled the discovery of potential virulence factors in the genome. Moreover, 14 proteins that may be involved in M. synoviae infection were identified, providing candidate proteins and facilitating the exploration of the infection mechanism of M. synoviae.
摘要：
鸡滑膜支原体感染率在全球范围内不断增加。基因组研究大大提高了我们对滑膜分枝杆菌生物学和毒力的理解。然而,大约20%的预测蛋白质具有未知的功能。特别是,滑膜分枝杆菌ATCC25204基因组有663个编码DNA序列，其中155个被认为是编码假想蛋白(HP)。这些基因中的几个可能编码未知的毒力因子。这项研究旨在重新注释滑膜分枝杆菌ATCC25204中的所有155种蛋白质，以使用当前可用的数据库和生物信息学工具来预测新的潜在毒力因子。最后,125个蛋白质被重新注释，包括酶（39%），脂蛋白（10%），DNA结合蛋白（6%），相变量血凝素（19%），和其他蛋白质类型（26％）。在155种蛋白质中，检测到28种与毒力相关的蛋白，其中五个被重新注释。此外，比较滑膜分枝杆菌感染细胞前后的HP表达，以鉴定潜在的毒力相关蛋白。14个HP基因表达上调,包括五个与毒力相关的基因。我们的研究将滑膜分枝杆菌ATCC25204的功能注释从76％提高到95％，并能够在基因组中发现潜在的毒力因子。此外，确定了可能与滑膜分枝杆菌感染有关的14种蛋白质，提供候选蛋白并促进滑膜分枝杆菌感染机制的探索。
