PDF(Pubmed)
Abstract：
Effective disinfection procedures in healthcare facilities are essential to prevent transmission. Chemical disinfectants, hydrogen peroxide vapour (HPV) systems and ultraviolet (UV) light are commonly used methods. An emerging method, violet-blue light at 405 nm, has shown promise for surface disinfection. Its antimicrobial properties are based on producing reactive oxygen species (ROS) that lead to the inactivation of pathogens. Studies have shown significant efficacy in reducing bacterial levels on surfaces and in the air, reducing nosocomial infections. The aim of this study was to evaluate the antimicrobial effectiveness of violet-blue (405 nm) LED lamps on high-contact surfaces in a hospital infection-control laboratory. High-contact surfaces were sampled before and after 7 days of exposure to violet-blue light. In addition, the effect of violet-blue light on MRSA-contaminated surfaces was investigated. Exposure to violet-blue light significantly reduced the number of bacteria, yeasts and moulds on the sampled surfaces. The incubator handle showed a low microbial load and no growth after irradiation. The worktable and sink showed an inconsistent reduction due to shaded areas. In the second experiment, violet-blue light significantly reduced the microbial load of MRSA on surfaces, with a greater reduction on steel surfaces than on plastic surfaces. Violet-blue light at 405 nm has proven to be an effective tool for pathogen inactivation in healthcare settings Violet-blue light shows promise as an additional and integrated tool to reduce microbial contamination in hospital environments but must be used in combination with standard cleaning practices and infection control protocols. Further research is needed to optimise the violet-blue, 405 nm disinfection method.
摘要：
医疗机构的有效消毒程序对于防止传播至关重要。化学消毒剂,过氧化氢蒸汽(HPV)系统和紫外(UV)光是常用的方法。一种新兴的方法，405nm的紫蓝光，已显示出表面消毒的希望。其抗微生物特性基于产生导致病原体失活的活性氧(ROS)。研究表明，在减少表面和空气中的细菌水平方面具有显着的功效，减少医院感染。这项研究的目的是在医院感染控制实验室中评估高接触表面上紫蓝色（405nm）LED灯的抗菌效果。在暴露于紫蓝光7天之前和之后对高接触表面进行采样。此外,研究了紫蓝光对MRSA污染表面的影响。暴露在紫蓝光下显著减少了细菌的数量，取样表面上的酵母和霉菌。培养箱手柄显示出微生物负荷低，辐照后无生长。由于阴影区域，工作台和水槽显示出不一致的减少。在第二个实验中,紫蓝光显著降低了MRSA在表面的微生物负荷，与一个更大的减少在钢表面比在塑料表面。405nm的紫蓝光已被证明是医疗保健环境中病原体灭活的有效工具紫蓝光有望作为减少医院环境中微生物污染的附加和集成工具，但必须与标准清洁实践和感染控制措施结合使用。需要进一步的研究来优化紫蓝色，405nm的消毒方法。
