PDF(Pubmed)
Abstract：
The coffee industry faces coffee leaf rust caused by Hemileia vastratix, which is considered the most devastating disease of the crop, as it reduces the photosynthetic rate and limits productivity. The use of plant resistance inducers, such as chitosan, is an alternative for the control of the disease by inducing the synthesis of phytoalexins, as well as the activation of resistance genes. Previously, the effect of chitosan from different sources and physicochemical properties was studied; however, its mechanisms of action have not been fully elucidated. In this work, the ability of food-grade high-density chitosan (0.01% and 0.05%) to control the infection caused by the pathogen was evaluated. Subsequently, the effect of high-density chitosan (0.05%) on the induction of pathogenesis-related gene expression (GLUC, POX, PAL, NPR1, and CAT), the enzymatic activity of pathogenesis-related proteins (GLUC, POX, SOD, PPO, and APX), and phytoalexin production were evaluated. The results showed that 0.05% chitosan increased the activity and gene expression of ß-1,3 glucanases and induced a differentiated response in enzymes related to the antioxidant system of plants. In addition, a correlation was observed between the activities of polyphenol oxidase and the production of phytoalexin, which allowed an effective defense response in coffee plants.
摘要：
咖啡行业面临由Hemileiavastratix引起的咖啡叶锈病，这被认为是农作物中最具破坏性的疾病，因为它降低了光合速率并限制了生产力。植物抗性诱导剂的使用，如壳聚糖，是一种通过诱导植物抗毒素的合成来控制疾病的替代方法，以及抗性基因的激活。以前,研究了不同来源壳聚糖的作用和理化性质；然而，其作用机制尚未完全阐明。在这项工作中,评估了食品级高密度壳聚糖（0.01％和0.05％）控制病原体引起的感染的能力。随后，高密度壳聚糖（0.05％）对诱导发病相关基因表达的影响（GLUC，POX,PAL,NPR1和CAT)，发病相关蛋白的酶活性(GLUC，POX,SOD,PPO,和APX），和植物抗毒素的生产进行了评估。结果表明，0.05％的壳聚糖增加了β-1,3葡聚糖酶的活性和基因表达，并诱导了与植物抗氧化系统相关的酶的分化反应。此外,观察到多酚氧化酶的活性与植物抗毒素的产生之间存在相关性，这使得咖啡植物产生了有效的防御反应。
