PDF(Pubmed)
Abstract：
The β-amylase (BAM) gene family encodes important enzymes that catalyze the conversion of starch to maltose in various biological processes of plants and play essential roles in regulating the growth and development of multiple plants. So far, BAMs have been extensively studied in Arabidopsis thaliana (A. thaliana). However, the characteristics of the BAM gene family in the crucial economic crop, cotton, have not been reported. In this study, 27 GhBAM genes in the genome of Gossypium hirsutum L (G. hirsutum) were identified by genome-wide identification, and they were divided into three groups according to sequence similarity and phylogenetic relationship. The gene structure, chromosome distribution, and collinearity of all GhBAM genes identified in the genome of G. hirsutum were analyzed. Further sequence alignment of the core domain of glucosyl hydrolase showed that all GhBAM family genes had the glycosyl hydrolase family 14 domain. We identified the BAM gene GhBAM7 and preliminarily investigated its function by transcriptional sequencing analysis, qRT-PCR, and subcellular localization. These results suggested that the GhBAM7 gene may influence fiber strength during fiber development. This systematic analysis provides new insight into the transcriptional characteristics of BAM genes in G. hirsutum. It may lay the foundation for further study of the function of these genes.
摘要：
β-淀粉酶（BAM）基因家族编码重要的酶，在植物的各种生物过程中催化淀粉转化为麦芽糖，并在调节多种植物的生长发育中起重要作用。到目前为止,已在拟南芥中广泛研究了BAM（A.thaliana）。然而,关键经济作物中BAM基因家族的特征，棉花,尚未报告。在这项研究中,陆地棉基因组中的27个GhBAM基因（G。hirsutum)通过全基因组鉴定来鉴定，根据序列相似性和系统发育关系分为三组。基因结构，染色体分布,并分析了陆地棉基因组中鉴定出的所有GhBAM基因的共线性。葡萄糖基水解酶核心结构域的进一步序列比对显示所有GhBAM家族基因都具有糖基水解酶家族14结构域。我们鉴定了BAM基因GhBAM7，并通过转录测序分析初步研究了其功能，qRT-PCR,和亚细胞定位。这些结果表明，GhBAM7基因可能会影响纤维发育过程中的纤维强度。这种系统分析为陆地棉中BAM基因的转录特征提供了新的见解。为进一步研究这些基因的功能奠定了基础。
