PDF(Pubmed)
Abstract：
OBJECTIVE: The gut microbial composition has been linked to metabolic and autoimmune diseases, including arthritis. However, there is a dearth of knowledge on the gut bacteriome, mycobiome, and virome in patients with gouty arthritis (GA).
METHODS: We conducted a comprehensive analysis of the multi-kingdom gut microbiome of 26 GA patients and 28 healthy controls, using whole-metagenome shotgun sequencing of their stool samples.
RESULTS: Profound alterations were observed in the gut bacteriome, mycobiome, and virome of GA patients. We identified 1,117 differentially abundant bacterial species, 23 fungal species, and 4,115 viral operational taxonomic units (vOTUs). GA-enriched bacteria included Escherichia coli_D GENOME144544, Bifidobacterium infantis GENOME095938, Blautia_A wexlerae GENOME096067, and Klebsiella pneumoniae GENOME147598, while control-enriched bacteria comprised Faecalibacterium prausnitzii_G GENOME147678, Agathobacter rectalis GENOME143712, and Bacteroides_A plebeius_A GENOME239725. GA-enriched fungi included opportunistic pathogens like Cryptococcus neoformans GCA_011057565, Candida parapsilosis GCA_000182765, and Malassezia spp., while control-enriched fungi featured several Hortaea werneckii subclades and Aspergillus fumigatus GCA_000002655. GA-enriched vOTUs mainly attributed to Siphoviridae, Myoviridae, Podoviridae, and Microviridae, whereas control-enriched vOTUs spanned 13 families, including Siphoviridae, Myoviridae, Podoviridae, Quimbyviridae, Phycodnaviridae, and crAss-like. A co-abundance network revealed intricate interactions among these multi-kingdom signatures, signifying their collective influence on the disease. Furthermore, these microbial signatures demonstrated the potential to effectively discriminate between patients and controls, highlighting their diagnostic utility.
CONCLUSIONS: This study yields crucial insights into the characteristics of the GA microbiota that may inform future mechanistic and therapeutic investigations.
METHODS: We conducted a comprehensive analysis of the multi-kingdom gut microbiome of 26 GA patients and 28 healthy controls, using whole-metagenome shotgun sequencing of their stool samples.
RESULTS: Profound alterations were observed in the gut bacteriome, mycobiome, and virome of GA patients. We identified 1,117 differentially abundant bacterial species, 23 fungal species, and 4,115 viral operational taxonomic units (vOTUs). GA-enriched bacteria included Escherichia coli_D GENOME144544, Bifidobacterium infantis GENOME095938, Blautia_A wexlerae GENOME096067, and Klebsiella pneumoniae GENOME147598, while control-enriched bacteria comprised Faecalibacterium prausnitzii_G GENOME147678, Agathobacter rectalis GENOME143712, and Bacteroides_A plebeius_A GENOME239725. GA-enriched fungi included opportunistic pathogens like Cryptococcus neoformans GCA_011057565, Candida parapsilosis GCA_000182765, and Malassezia spp., while control-enriched fungi featured several Hortaea werneckii subclades and Aspergillus fumigatus GCA_000002655. GA-enriched vOTUs mainly attributed to Siphoviridae, Myoviridae, Podoviridae, and Microviridae, whereas control-enriched vOTUs spanned 13 families, including Siphoviridae, Myoviridae, Podoviridae, Quimbyviridae, Phycodnaviridae, and crAss-like. A co-abundance network revealed intricate interactions among these multi-kingdom signatures, signifying their collective influence on the disease. Furthermore, these microbial signatures demonstrated the potential to effectively discriminate between patients and controls, highlighting their diagnostic utility.
CONCLUSIONS: This study yields crucial insights into the characteristics of the GA microbiota that may inform future mechanistic and therapeutic investigations.
摘要：
目的：肠道微生物组成与代谢和自身免疫性疾病有关，包括关节炎.然而,对肠道细菌组缺乏了解，mycobiome,痛风性关节炎（GA）患者的病毒血症。
方法：我们对26名GA患者和28名健康对照者的多重肠道菌群进行了综合分析，使用全基因组鸟枪测序他们的粪便样本。
结果：在肠道细菌组中观察到明显的改变，mycobiome,和GA患者的病毒。我们确定了1,117种差异丰富的细菌，23种真菌，和4,115个病毒操作分类单位(vOTU)。富含GA的细菌包括大肠杆菌_DGENOME144544，婴儿双歧杆菌GENOME095938，Blautia_AwexleraeGENOME096067和肺炎克雷伯菌GENOME147598，而富含对照的细菌包括普氏粪杆菌_GGENOME147678，GENOME23Bacters-rect_O97ble233712富含GA的真菌包括机会性病原体，例如新生隐球菌GCA_011057565，近带念珠菌GCA_000182765和马拉色菌。,而富含对照的真菌则具有多个百叶werneckii亚进化枝和烟曲霉GCA_000002655。富含GA的vOTUs主要归因于虹彩病毒科，Myoviridae,波多病毒科,和微病毒科，而控制丰富的vOTU跨越13个家庭，包括硅藻科,Myoviridae,波多病毒科,昆比病毒科,Phycodnaviridae,和crass一样。一个共同丰富的网络揭示了这些多王国签名之间错综复杂的相互作用，表明他们对疾病的集体影响。此外，这些微生物特征证明了有效区分患者和对照组的潜力，强调他们的诊断效用。
结论：这项研究对GA微生物群的特征产生了重要的见解，这可能会为未来的机理和治疗研究提供信息。
方法：我们对26名GA患者和28名健康对照者的多重肠道菌群进行了综合分析，使用全基因组鸟枪测序他们的粪便样本。
结果：在肠道细菌组中观察到明显的改变，mycobiome,和GA患者的病毒。我们确定了1,117种差异丰富的细菌，23种真菌，和4,115个病毒操作分类单位(vOTU)。富含GA的细菌包括大肠杆菌_DGENOME144544，婴儿双歧杆菌GENOME095938，Blautia_AwexleraeGENOME096067和肺炎克雷伯菌GENOME147598，而富含对照的细菌包括普氏粪杆菌_GGENOME147678，GENOME23Bacters-rect_O97ble233712富含GA的真菌包括机会性病原体，例如新生隐球菌GCA_011057565，近带念珠菌GCA_000182765和马拉色菌。,而富含对照的真菌则具有多个百叶werneckii亚进化枝和烟曲霉GCA_000002655。富含GA的vOTUs主要归因于虹彩病毒科，Myoviridae,波多病毒科,和微病毒科，而控制丰富的vOTU跨越13个家庭，包括硅藻科,Myoviridae,波多病毒科,昆比病毒科,Phycodnaviridae,和crass一样。一个共同丰富的网络揭示了这些多王国签名之间错综复杂的相互作用，表明他们对疾病的集体影响。此外，这些微生物特征证明了有效区分患者和对照组的潜力，强调他们的诊断效用。
结论：这项研究对GA微生物群的特征产生了重要的见解，这可能会为未来的机理和治疗研究提供信息。
