PDF(Pubmed)
Abstract：
The world population is experiencing colossal growth and thus demand for food, leading to an increase in the use of pesticides. Persistent pesticide contamination, such as carbendazim, remains a pressing environmental concern, with potentially long-term impacts on aquatic ecosystems. In the present study, Daphnia magna was exposed to carbendazim (5 µg L-1) for 12 generations, with the aim of assessing gene transcription alterations induced by carbendazim (using a D. magna custom microarray). The results showed that carbendazim caused changes in genes involved in the response to stress, DNA replication/repair, neurotransmission, ATP production, and lipid and carbohydrate metabolism at concentrations already found in the environment. These outcomes support the results of previous studies, in which carbendazim induced genotoxic effects and reproduction impairment (increasing the number of aborted eggs with the decreasing number of neonates produced). The exposure of daphnids to carbendazim did not cause a stable change in gene transcription between generations, with more genes being differentially expressed in the F0 generation than in the F12 generation. This could show some possible daphnid acclimation after 12 generations and is aligned with previous multigenerational studies where few ecotoxicological effects at the individual and populational levels and other subcellular level effects (e.g., biochemical biomarkers) were found.
摘要：
世界人口正在经历巨大的增长，因此对粮食的需求，导致农药使用量的增加。持续的农药污染，比如多菌灵,仍然是一个紧迫的环境问题，对水生生态系统有潜在的长期影响。在本研究中,大型水蚤暴露于多菌灵（5µgL-1）12代，目的是评估多菌灵诱导的基因转录改变(使用D.magna定制微阵列)。结果表明，多菌灵引起了参与应激反应的基因的变化，DNA复制/修复，神经传递,ATP生产，以及环境中已经发现的浓度的脂质和碳水化合物代谢。这些结果支持了先前研究的结果，其中多菌灵引起遗传毒性作用和生殖障碍（随着新生儿数量的减少，流产卵的数量增加）。暴露于多菌灵的水蚤并没有引起世代之间基因转录的稳定变化，与F12代相比，F0代差异表达的基因更多。这可能显示出12代后的一些可能的daphnid适应，并且与先前的多代研究一致，这些研究在个体和人群水平上几乎没有生态毒理学影响以及其他亚细胞水平的影响（例如，生化生物标志物）被发现。
