PDF(Pubmed)
Abstract：
The cotton fleahopper (Pseudatomoscelis seriatus Reuter) is considered a highly economically damaging pest of cotton (Gossypium hirsutum L.) in Texas and Oklahoma. Current control methods rely heavily on the use of foliar-applied chemical insecticides, but considering the cost of insecticides and the critical timeliness of applications, chemical control methods are often not optimized to reduce potential yield losses from this pest. The Bacillus thuringiensis (Bt) Mpp51Aa2 (formerly Cry51Aa2.834_16) protein has proven effective against thrips and plant bugs with piercing and sucking feeding behaviors, but the impact of this toxin on cotton fleahoppers has not been investigated. To evaluate the Mpp51Aa2 trait effectiveness towards the cotton fleahopper, field trials were conducted in 2019, 2020, and 2021, comparing a cotton cultivar containing the Mpp51Aa2 trait to a non-traited isoline cultivar under insecticide-treated and untreated conditions. Populations of cotton fleahopper nymphs and adults were estimated weekly by visually inspecting cotton terminals. Square retention was also assessed during the first week of bloom to provide some insight on how the Bt trait may influence yield. While cotton fleahopper population differences between the traited and non-traited plants were not consistently noted during the pre-bloom squaring period, there was a consistent increase in square retention in cotton expressing Mpp51Aa2 relative to non-traited cotton. Additionally, cotton expressing Mpp51Aa2 offered similar square protection relative to non-traited cotton treated with insecticides for the cotton fleahopper. These findings indicate that the Mpp51Aa2 protein should provide benefits of delayed nymphal growth, population suppression, and increased square retention.
摘要：
在德克萨斯州和俄克拉荷马州，棉花跳蚤（PseudatomoscelisseriatusReuter）被认为是对棉花（GossypiumhirsutumL.）具有高度经济破坏性的害虫。当前的控制方法严重依赖于使用叶面施用的化学杀虫剂，但是考虑到杀虫剂的成本和应用的关键及时性，化学控制方法通常没有优化以减少该害虫的潜在产量损失。苏云金芽孢杆菌（Bt）Mpp51Aa2（以前称为Cry51Aa2.834_16）蛋白已被证明对thrips和植物虫子有效，具有刺穿和吸食行为，但是这种毒素对棉花跳蚤的影响尚未得到研究。为了评估Mpp51Aa2对棉花跳蚤的性状有效性，在2019年，2020年和2021年进行了田间试验，将含有Mpp51Aa2性状的棉花品种与在杀虫剂处理和未经处理的条件下的非遗传等值线品种进行了比较。通过目视检查棉花终端，每周估计棉花跳蚤若虫和成虫的种群。在开花的第一周还评估了正方形保留，以提供有关Bt性状如何影响产量的一些见解。虽然在开花前的平方期没有一致地注意到成虫和非成虫之间的棉花跳蚤种群差异，相对于未训练的棉花，表达Mpp51Aa2的棉花的平方保留率持续增加。此外,表达Mpp51Aa2的棉花相对于用杀虫剂处理过的棉花为棉花跳蚤提供了类似的正方形保护。这些发现表明，Mpp51Aa2蛋白应提供延迟若虫生长的益处，人口压制，并增加了正方形保留。
