PDF(Pubmed)
Abstract：
Wound infection is a common complication of chronic wounds. It can impair healing, which may not occur without external help. Antimicrobial dressings (AMDs) are a type of external help to infected chronic wounds. In this study, highly porous membranes made of only chitosan and containing the antiseptic polyhexanide (poly(hexamethylene biguanide); PHMB) were prepared by cryogelation, aiming to be used in AMDs. These membranes exhibited a water swelling capacity of 748%, a water drop penetration time of 11 s in a dry membrane and a water vapor transmission rate of 34,400 g H2O/m2/24 h when in contact with water. The best drug loading method involved simultaneous loading by soaking in a PHMB solution and sterilization by autoclaving, resulting in sterilized, drug-loaded membranes. When these membranes and a commercial PHMB-releasing AMD were assayed under the same conditions, albeit far from the in vivo conditions, their drug release kinetics were comparable, releasing PHMB for ca. 6 and 4 h, respectively. These membranes exhibited high antibacterial activity against Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa, which are bacterial species commonly found in infected wounds and blood clotting activity. The obtained results suggest that these membranes may have potential for use in the development of AMDs.
摘要：
伤口感染是慢性伤口的常见并发症。它会损害愈合，如果没有外部帮助，这可能不会发生。抗菌敷料（AMD）是一种外部帮助感染的慢性伤口。在这项研究中,通过冷冻凝胶法制备了仅由壳聚糖制成的高度多孔膜，其中含有抗菌聚己内酯（聚（六亚甲基双胍）；PHMB），旨在用于AMD。这些膜表现出748%的水溶胀能力,与水接触时,水滴在干膜中的渗透时间为11s,水蒸气透过率为34,400gH2O/m2/24h。最佳的药物装载方法包括通过浸泡在PHMB溶液中同时装载和通过高压灭菌灭菌，导致灭菌，载药膜.当这些膜和市售的释放PHMB的AMD在相同的条件下测定时，尽管远离体内条件，它们的药物释放动力学相当，释放PHMB的CA。6和4小时，分别。这些膜对大肠杆菌表现出很高的抗菌活性，金黄色葡萄球菌和铜绿假单胞菌,这是细菌物种通常发现在感染的伤口和血液凝固活动。获得的结果表明，这些膜可能具有用于开发AMD的潜力。
