PDF(Pubmed)
Abstract：
Development of biocomposite scaffolds has gained tremendous attention due to their potential for tissue regeneration. However, most scaffolds often contain animal-derived collagen that may elicit an immunological response, necessitating the development of new biomaterials. Herein, we developed a new collagen-like peptide,(Pro-Ala-His)10 (PAH)10, and explored its ability to be utilized as a functional biomaterial by incorporating it with a newly synthesized peptide-based self-assembled gel. The gel was prepared by conjugating a pectin derivative, galataric acid, with a pro-angiogenic peptide (LHYQDLLQLQY) and further functionalized with a cortistatin-derived peptide, (Phe-Trp-Lys-Thr)4 (FWKT)4, and the bio-ionic liquid choline acetate. The self-assembly of (PAH)10 and its interactions with the galactarate-peptide conjugates were examined using replica exchange molecular dynamics (REMD) simulations. Results revealed the formation of a multi-layered scaffold, with enhanced stability at higher temperatures. We then synthesized the scaffold and examined its physicochemical properties and its ability to integrate with aortic smooth muscle cells. The scaffold was further utilized as a bioink for bioprinting to form three-dimensional cell-scaffold matrices. Furthermore, the formation of actin filaments and elongated cell morphology was observed. These results indicate that the (PAH)10 hybrid scaffold provides a suitable environment for cell adhesion, proliferation and growth, making it a potentially valuable biomaterial for tissue engineering.
摘要：
生物复合材料支架的开发由于其组织再生的潜力而获得了极大的关注。然而,大多数支架通常含有动物来源的胶原蛋白，可以引起免疫反应，需要开发新的生物材料。在这里，我们开发了一种新的胶原样肽，（Pro-Ala-His）10（PAH）10，并通过将其与新合成的基于肽的自组装凝胶相结合来探索其用作功能性生物材料的能力。通过缀合果胶衍生物制备凝胶，戊二酸,用促血管生成肽（LHYQDLLQLQQY）和皮质抑素衍生肽进一步功能化，(Phe-Trp-Lys-Thr)4(FWKT)4和生物离子液体胆碱乙酸酯。使用复制交换分子动力学（REMD）模拟检查（PAH）10的自组装及其与半乳糖酸-肽缀合物的相互作用。结果显示多层支架的形成，在较高的温度下具有增强的稳定性。然后，我们合成了支架，并检查了其理化性质及其与主动脉平滑肌细胞整合的能力。支架进一步用作生物打印的生物墨水以形成三维细胞支架基质。此外，观察到肌动蛋白丝的形成和细长的细胞形态。这些结果表明，（PAH）10混合支架为细胞粘附提供了合适的环境，增殖和生长，使其成为组织工程的潜在有价值的生物材料。
