PDF(Pubmed)
Abstract：
Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola, collectively recognized as periodontopathogens within the red complex, have been extensively studied in clinical samples collected from individuals with periodontitis. A lab-on-a-chip (LOC) is a miniature mechanism that integrates various laboratory operations onto a single microchip or a small-scale platform. This systematic review evaluates the application of LOC technology in identifying microorganisms from the red complex. This study adhered to PRISMA recommendations, and the review process encompassed several databases. In the electronic search, a total of 58 reports were found, and ultimately, 10 studies were considered relevant for inclusion. All these studies described effective, rapid, and reliable LOC systems for detecting and amplifying P. gingivalis, T. forsythia, and T. denticola. Compared to traditional methods, the LOC approach demonstrated minimal reagent requirements. Additionally, the results indicated that the amplification process took approximately 2 to 8 min, while detection could be completed in as little as 2 min and 40 s, resulting in a total experimental duration of around 11 min. Integrating miniaturization, speed, accuracy, and automation within microchip platforms makes them promising tools for detecting and amplifying microorganisms associated with the red complex in periodontal diseases.
摘要：
牙龈卟啉单胞菌,连翘坦菌,和Denticola密螺旋体,在红色复合物中被统称为牙周病原体，在从牙周炎患者收集的临床样本中进行了广泛的研究。芯片实验室（LOC）是一种微型机制，可将各种实验室操作集成到单个微芯片或小规模平台上。本系统评价了LOC技术在从红色复合物中鉴定微生物中的应用。这项研究遵循了PRISMA的建议，审查过程包括几个数据库。在电子搜索中，共发现58份报告，最终,10项研究被认为与纳入相关。所有这些研究都描述了有效的，快速,和可靠的LOC系统，用于检测和扩增牙龈卟啉单胞菌，T.连翘,还有T.Denticola.与传统方法相比，LOC方法显示最低的试剂要求。此外,结果表明，扩增过程大约需要2到8分钟，虽然检测可以在短短2分钟和40秒内完成，导致约11分钟的总实验持续时间。集成小型化，速度,准确度,微芯片平台内的自动化使它们成为检测和扩增与牙周病中红色复合物相关的微生物的有前途的工具。
