PDF(Pubmed)
Abstract：
Fungi play key roles in forest soils and provide benefits to trees via mycorrhizal symbioses. After severe disturbance, forest regrowth can be impeded because of changes in fungal communities. In 2013-2014, soil fungi in forest floor and mineral soil were examined by Roche 454 pyrosequencing in undisturbed, harvested, and burned jack pine stands in a forested area near Fort Chipewyan, Alberta. These fungal communities were compared with jack pine, white spruce, and larch stands in Gateway Hill, a nearby certified reclaimed area. In 2014, a more detailed sampling of forestry and reclamation jack pine sites examined fungi in soil fractions using two high-throughput sequencing platforms and a sporocarp survey. The significances of compositional and functional differences in fungal communities between the forested and reclamation sites were assessed using permutation tests of partially constrained ordinations, accounting for confounding factors by variance partitioning. Taxa associated with the forestry area were primarily ectomycorrhizal. Fungal richness and diversity were greater in soils from the reclamation sites and included significantly more pathogenic taxa and taxa with unknown functional properties. Fungal community dissimilarities may have been artefacts of historical legacies or, alternatively, may have resulted from contrasting niche differentiation between forestry and reclamation sites.
摘要：
真菌在森林土壤中起着关键作用，并通过菌根共生为树木提供利益。在严重的骚乱之后,由于真菌群落的变化，森林的再生可能会受到阻碍。在2013-2014年，通过Roche454焦磷酸测序法检查了林地和矿质土壤中的土壤真菌，收获,在Chipewyan堡附近的森林地区烧毁了杰克松树林，艾伯塔省.将这些真菌群落与杰克松进行了比较，白色云杉,落叶松在盖特威山，附近的一个经过认证的填海区域。2014年，对林业和开垦的杰克松站点进行了更详细的采样，使用两个高通量测序平台和一个子果皮调查检查了土壤部分中的真菌。使用部分受约束的纵坐标的置换检验评估了森林和开垦地点之间真菌群落的组成和功能差异的重要性，通过方差划分来考虑混杂因素。与林区相关的类群主要是外生菌根。开垦地点土壤中的真菌丰富度和多样性更高，并且包括更多的病原类群和功能特性未知的类群。真菌社区的差异可能是历史遗产的文物，或者，可能是由于林业和垦区之间的生态位差异形成的。
