PDF(Pubmed)
Abstract：
It has been repeatedly reported that the cells of organisms in all kingdoms of life produce nanometer-sized lipid membrane-enveloped extracellular vesicles (EVs), transporting and protecting various substances of cellular origin. While the composition of EVs produced by human pathogenic fungi has been studied in recent decades, another important challenge is the analysis of their functionality. Thus far, fungal EVs have been shown to play significant roles in intercellular communication, biofilm production, and modulation of host immune cell responses. In this study, we verified the involvement of biofilm-derived EVs produced by two different strains of Candida albicans-C. albicans SC5314 and 3147 (ATCC 10231)-in various aspects of biofilm function by examining its thickness, stability, metabolic activity, and cell viability in the presence of EVs and the antifungal drug caspofungin. Furthermore, the proteolytic activity against the kininogen-derived antimicrobial peptide NAT26 was confirmed by HPLC analysis for C. albicans EVs that are known to carry, among others, particular members of the secreted aspartic proteinases (Saps) family. In conclusion, EVs derived from C. albicans biofilms were shown to be involved in biofilm tolerance to caspofungin, biofilm detachment, and fungal proteolytic activity.
摘要：
它已被反复报道，在所有的生命王国的生物体的细胞产生纳米大小的脂质膜包裹的细胞外囊泡（EV），运输和保护细胞来源的各种物质。虽然近几十年来已经研究了人类病原真菌产生的电动汽车的组成,另一个重要的挑战是对其功能的分析。到目前为止,真菌EV已被证明在细胞间通讯中起重要作用，生物膜生产，和调节宿主免疫细胞反应。在这项研究中,我们验证了由两种不同的白色念珠菌-C菌株产生的生物膜衍生的EV的参与。白色念珠菌SC5314和3147（ATCC10231）-通过检查其厚度在生物膜功能的各个方面，稳定性,代谢活动，以及在有EVs和抗真菌药物卡泊芬净存在下的细胞活力。此外，对激肽原来源的抗菌肽NAT26的蛋白水解活性通过HPLC分析证实，其中,分泌的天冬氨酸蛋白酶（Saps）家族的特定成员。总之,来自白色念珠菌生物膜的电动汽车被证明参与了对卡泊芬净的生物膜耐受性，生物膜脱离,和真菌蛋白水解活性。
