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Abstract：
The role of microorganisms in effectively terminating harmful algal blooms (HABs) is crucial for maintaining environmental stability. Recent studies have placed increased emphasis on bio-agents capable of inhibiting HABs. The bacterium Pseudoalteromonas sp. strain FDHY-MZ2 has exhibited impressive algicidal abilities against Karenia mikimotoi, a notorious global HAB-forming species. To augment this capability, cultures were progressively scaled from shake flask conditions to small-scale (5 L) and pilot-scale (50 L) fermentation. By employing a specifically tailored culture medium (2216E basal medium with 1.5% soluble starch and 0.5% peptone), under precise conditions (66 h, 20 °C, 450 rpm, 30 L/min ventilation, 3% seeding, and constant starch flow), a notable increase in algicidal bacterial biomass was observed; the bacterial dosage required to entirely wipe out K. mikimotoi within a day decreased from 1% to 0.025%. Compared to an unoptimized shake flask group, the optimized fermentation culture caused significant reductions in algal chlorophyll and protein levels (21.85% and 78.3%, respectively). Co-culturing induced increases in algal malondialdehyde and H2O2 by 5.98 and 5.38 times, respectively, leading to further disruption of algal photosynthesis. This study underscores the unexplored potential of systematically utilized microbial agents in mitigating HABs, providing a pathway for their wider application.
摘要：
微生物在有效终止有害藻华（HAB）中的作用对于维持环境稳定性至关重要。最近的研究越来越重视能够抑制HAB的生物制剂。细菌假交替单胞菌属。菌株FDHY-MZ2对Kareniamikimootoi表现出令人印象深刻的杀藻能力，一个臭名昭著的全球HAB形成物种。为了增强这种能力，从摇瓶条件到小规模（5L）和中试规模（50L）发酵逐渐扩大培养物。通过使用专门定制的培养基（含1.5％可溶性淀粉和0.5％蛋白胨的2216E基础培养基），在精确条件下(66小时，20°C,450转/分,30L/min通风,3%播种,和恒定的淀粉流量)，观察到溶藻细菌生物量显着增加;在一天内完全消灭K.mikimotoi所需的细菌剂量从1％降至0.025％。与未优化的摇瓶组相比，优化的发酵培养导致藻类叶绿素和蛋白质水平显著降低(21.85%和78.3%，分别)。共培养诱导藻类丙二醛和H2O2增加5.98和5.38倍，分别,导致藻类光合作用的进一步破坏。这项研究强调了系统利用微生物制剂减轻HAB的未开发潜力，为其更广泛的应用提供了途径。
