Abstract：
Regeneration of lost body parts is a widespread phenomenon across annelids. However, the molecular inducers of the cell sources for this reparative morphogenesis have not been identified. We have identified a regeneration-related gene Oxfibrillin from the transcriptome analysis of a polychaeta, Ophryotrocha xiamen, which is found to be a well-suited model to study the mechanisms of regeneration. Fibrillins are large glycoproteins that assemble to form the microfibrils and regulate growth factors or other transfer processes. Here, we obtained the 31,274 bp genomic DNA sequences of Oxfibrillin. The coding sequence length was 5784 bp encoding 1927 amino acids with a VWD domain, EGF/cb-EGF domains, a TR domain, and a transmembrane domain. Oxfibrillin was positioned within the subgroup of invertebrates and showed low scores for homology to mammalian fibrillin. In gene expression analysis, Oxfibrillin genes were constantly upregulated during the early regeneration process and then remained stable until the formation of the complete tail which indicated that it might be a vital factor to affect posterior regeneration process. Therefore, the Oxfibrillin of O. xiamen might play important roles in the regeneration process.
摘要：
丢失的身体部位的再生是环节动物的普遍现象。然而,这种修复性形态发生的细胞来源的分子诱导物尚未被鉴定。我们已经从多毛囊的转录组分析中确定了与再生相关的基因Oxficloin，厦门Ophryotrocha,这是一个非常适合研究再生机制的模型。原纤维素是组装形成微纤维并调节生长因子或其他转移过程的大糖蛋白。这里,我们获得了氧纤蛋白31,274bp的基因组DNA序列。编码序列长度为5784bp，编码具有VWD结构域的1927个氨基酸，EGF/cb-EGF域，一个TR域，和跨膜结构域。氧纤蛋白位于无脊椎动物亚组中，与哺乳动物纤纤蛋白的同源性得分较低。在基因表达分析中，氧纤蛋白基因在早期再生过程中不断上调，然后保持稳定，直到形成完整的尾巴，这表明它可能是影响后继再生过程的重要因素。因此,厦门O.的氧纤蛋白可能在再生过程中起重要作用。
