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Abstract：
BACKGROUND: The influence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection on assisted reproductive technology (ART) has received increasing attention. It has been reported that the SARS-CoV-2 RiboNucleic Acid (RNA) cannot be detected in follicular fluid and granulosa cells. However, the detection rate of SARS-CoV-2 RNA in immature oocytes and blastocysts has still unknown. Moreover, the effect of SARS-CoV-2 infection on embryological outcomes in ART during the Omicron epidemic is limited.
METHODS: A prospective study was performed to explore the detection rate of viral RNA in biological specimens from patients who tested positive for SARS-CoV-2 RNA and the effects of SARS-CoV-2 infection on embryological outcomes. A total of 211 patients underwent transvaginal oocyte retrieval at the Third Affiliated Hospital of Guangzhou Medical University between December 13, 2022 and December 30, 2022. Prior to transvaginal oocyte retrieval, 61 individuals tested positive for SARS-CoV-2 RNA within 24 h. Follicular fluid was preserved during oocyte retrieval. Granular cells were collected after degranulation (Intracytoplasmic sperm injection only). Immature oocytes were collected at the end of the ICSI. Unavailable blastocysts were collected on day 6 (D6). The TIANLONG SARS-CoV-2 RT-PCR-Kit was used to detect SARS-CoV-2 RNA in all samples. The COVID-19 and Non COVID-19 groups were contrasted in the following areas: fertilization rate, 2PN rate, Day 3 (D3) available embryos rate, D3 good-quality embryos rate, blastocyst formation rate, good-quality blastocyst formation rate.
RESULTS: All samples were negative except for an immature oocytes sample that was positive for SARS-CoV-2 viral RNA with a detection rate of 6.67%. Whether in-vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI), the rate of fertilization, 2PN, D3 available embryos, D3 good-quality embryos, blastocyst formation, good-quality blastocyst formation was not significantly negative different between the COVID-19 and the Non COVID-19 groups. Our findings were validated by an overview of the embryological outcome from the cycles before SARS- Cov-2 infection from the same patient.
CONCLUSIONS: Except for immature oocytes, none of the follicular fluid, granulosa cells, or blastocysts samples contained viral RNA. In addition, SARS-CoV-2 infection had no detrimental effects on the embryological outcomes of ART.
METHODS: A prospective study was performed to explore the detection rate of viral RNA in biological specimens from patients who tested positive for SARS-CoV-2 RNA and the effects of SARS-CoV-2 infection on embryological outcomes. A total of 211 patients underwent transvaginal oocyte retrieval at the Third Affiliated Hospital of Guangzhou Medical University between December 13, 2022 and December 30, 2022. Prior to transvaginal oocyte retrieval, 61 individuals tested positive for SARS-CoV-2 RNA within 24 h. Follicular fluid was preserved during oocyte retrieval. Granular cells were collected after degranulation (Intracytoplasmic sperm injection only). Immature oocytes were collected at the end of the ICSI. Unavailable blastocysts were collected on day 6 (D6). The TIANLONG SARS-CoV-2 RT-PCR-Kit was used to detect SARS-CoV-2 RNA in all samples. The COVID-19 and Non COVID-19 groups were contrasted in the following areas: fertilization rate, 2PN rate, Day 3 (D3) available embryos rate, D3 good-quality embryos rate, blastocyst formation rate, good-quality blastocyst formation rate.
RESULTS: All samples were negative except for an immature oocytes sample that was positive for SARS-CoV-2 viral RNA with a detection rate of 6.67%. Whether in-vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI), the rate of fertilization, 2PN, D3 available embryos, D3 good-quality embryos, blastocyst formation, good-quality blastocyst formation was not significantly negative different between the COVID-19 and the Non COVID-19 groups. Our findings were validated by an overview of the embryological outcome from the cycles before SARS- Cov-2 infection from the same patient.
CONCLUSIONS: Except for immature oocytes, none of the follicular fluid, granulosa cells, or blastocysts samples contained viral RNA. In addition, SARS-CoV-2 infection had no detrimental effects on the embryological outcomes of ART.
摘要：
背景：严重急性呼吸综合征冠状病毒2（SARS-CoV-2）感染对辅助生殖技术（ART）的影响越来越受到关注。据报道，SARS-CoV-2核糖核酸（RNA）无法在卵泡液和颗粒细胞中检测到。然而,SARS-CoV-2RNA在未成熟卵母细胞和胚泡中的检出率尚不清楚。此外，在Omicron流行期间,SARS-CoV-2感染对ART胚胎结局的影响有限.
方法：进行了一项前瞻性研究，以探讨SARS-CoV-2RNA检测阳性的患者的生物标本中病毒RNA的检出率以及SARS-CoV-2感染对胚胎结局的影响。2022年12月13日至2022年12月30日，共有211例患者在广州医科大学附属第三医院接受了经阴道取卵术。在经阴道取卵前,61名个体在24小时内检测出SARS-CoV-2RNA呈阳性。在取卵过程中保留了卵泡液。在脱颗粒后收集颗粒细胞(仅卵胞浆内单精子注射)。在ICSI结束时收集未成熟卵母细胞。在第6天(D6)收集不可用的胚泡。使用天龙SARS-CoV-2RT-PCR-试剂盒检测所有样品中的SARS-CoV-2RNA。COVID-19和非COVID-19组在以下方面进行了对比：受精率，2PN率，第3天（D3）可用胚胎率，D3优质胚胎率，囊胚形成率,优质囊胚形成率。
结果：除未成熟卵母细胞样本SARS-CoV-2病毒RNA阳性外，其余样本均为阴性，检出率为6.67%。无论是体外受精（IVF）还是卵胞浆内单精子注射（ICSI），受精率,2PN,D3可用胚胎，D3优质胚胎，囊胚形成,COVID-19组和非COVID-19组之间的优质胚泡形成无明显阴性差异。我们的发现通过对来自同一患者的SRAS-Cov-2感染前周期的胚胎学结果的概述得到了验证。
结论：除未成熟卵母细胞外，没有卵泡液,颗粒细胞,或胚泡样品含有病毒RNA。此外,SARS-CoV-2感染对ART的胚胎结局没有不利影响。
方法：进行了一项前瞻性研究，以探讨SARS-CoV-2RNA检测阳性的患者的生物标本中病毒RNA的检出率以及SARS-CoV-2感染对胚胎结局的影响。2022年12月13日至2022年12月30日，共有211例患者在广州医科大学附属第三医院接受了经阴道取卵术。在经阴道取卵前,61名个体在24小时内检测出SARS-CoV-2RNA呈阳性。在取卵过程中保留了卵泡液。在脱颗粒后收集颗粒细胞(仅卵胞浆内单精子注射)。在ICSI结束时收集未成熟卵母细胞。在第6天(D6)收集不可用的胚泡。使用天龙SARS-CoV-2RT-PCR-试剂盒检测所有样品中的SARS-CoV-2RNA。COVID-19和非COVID-19组在以下方面进行了对比：受精率，2PN率，第3天（D3）可用胚胎率，D3优质胚胎率，囊胚形成率,优质囊胚形成率。
结果：除未成熟卵母细胞样本SARS-CoV-2病毒RNA阳性外，其余样本均为阴性，检出率为6.67%。无论是体外受精（IVF）还是卵胞浆内单精子注射（ICSI），受精率,2PN,D3可用胚胎，D3优质胚胎，囊胚形成,COVID-19组和非COVID-19组之间的优质胚泡形成无明显阴性差异。我们的发现通过对来自同一患者的SRAS-Cov-2感染前周期的胚胎学结果的概述得到了验证。
结论：除未成熟卵母细胞外，没有卵泡液,颗粒细胞,或胚泡样品含有病毒RNA。此外,SARS-CoV-2感染对ART的胚胎结局没有不利影响。
