Abstract：
OBJECTIVE: To investigate the effect of epithelial growth factor (EGF) with collagen matrix (CM) on the gain of KT for buccally positioned implants in dogs.
METHODS: In five dogs, four implants were placed buccally with the whole part of KT excision on the buccal side (two implants per each hemi-mandible). After one month, KT augmentation was performed: 1) free gingival grafts (FGG), 2) collagen matrix (CM) only, 3) CM soaked with 1 μg/g of EGF, and 4) CM soaked with 10 μg/g of EGF (n = 5 in each group). The experimental animals were sacrificed three months post-KT augmentation. Clinical, histologic, and histomorphometric analyses were performed.
RESULTS: The clinical KT zone was the highest in group FGG (5.16 ± 1.63 mm). Histologically, all groups presented buccal bony dehiscence. Regarding newly formed KT, no specific difference was found among the groups, but robust rete pegs formation in some specimens in group FGG. Histomorphometric KT height (4.66 ± 1.81 mm) and length (5.56 ± 2.25 mm) were the highest in group FGG, whereas similar increases were noted in the rest. The buccal soft tissue thickness at the coronal part of the implant did not exceed 2 mm in all groups.
CONCLUSIONS: All groups presented increased KT zone, but FGG treatment was more favored. The addition of EGF to CM appeared not to enhance KT formation.
CONCLUSIONS: FGG treatment was more favorable to re-establish the KT zone than other treatment modalities.
METHODS: In five dogs, four implants were placed buccally with the whole part of KT excision on the buccal side (two implants per each hemi-mandible). After one month, KT augmentation was performed: 1) free gingival grafts (FGG), 2) collagen matrix (CM) only, 3) CM soaked with 1 μg/g of EGF, and 4) CM soaked with 10 μg/g of EGF (n = 5 in each group). The experimental animals were sacrificed three months post-KT augmentation. Clinical, histologic, and histomorphometric analyses were performed.
RESULTS: The clinical KT zone was the highest in group FGG (5.16 ± 1.63 mm). Histologically, all groups presented buccal bony dehiscence. Regarding newly formed KT, no specific difference was found among the groups, but robust rete pegs formation in some specimens in group FGG. Histomorphometric KT height (4.66 ± 1.81 mm) and length (5.56 ± 2.25 mm) were the highest in group FGG, whereas similar increases were noted in the rest. The buccal soft tissue thickness at the coronal part of the implant did not exceed 2 mm in all groups.
CONCLUSIONS: All groups presented increased KT zone, but FGG treatment was more favored. The addition of EGF to CM appeared not to enhance KT formation.
CONCLUSIONS: FGG treatment was more favorable to re-establish the KT zone than other treatment modalities.
摘要：
目的：探讨上皮生长因子（EGF）和胶原基质（CM）对犬口腔植入KT增加的影响。
方法：在五只狗中，将四个植入物经颊放置，将KT切除的整个部分放在颊侧（每个半下颌骨两个植入物）。一个月后,进行KT增强：1）游离牙龈移植物（FGG），2）胶原基质（CM），3)CM浸泡1μg/g的EGF,和4)用10μg/gEGF浸泡的CM(每组n=5)。在KT增强后三个月处死实验动物。临床,组织学，并进行了组织形态计量学分析。
结果：FGG组临床KT区最高(5.16±1.63mm)。组织学上,所有组均出现颊骨裂开。关于新成立的KT，组间没有发现具体差异，但在FGG组的一些标本中形成了坚固的网钉。组织形态KT高度(4.66±1.81mm)和长度(5.56±2.25mm)在FGG组最高,而其余的则有类似的增加。在所有组中，植入物冠状部分的颊软组织厚度均不超过2mm。
结论：所有组的KT区增加，但FGG治疗更受青睐。向CM中添加EGF似乎不增强KT形成。
结论：FGG治疗比其他治疗方式更有利于重建KT区。
方法：在五只狗中，将四个植入物经颊放置，将KT切除的整个部分放在颊侧（每个半下颌骨两个植入物）。一个月后,进行KT增强：1）游离牙龈移植物（FGG），2）胶原基质（CM），3)CM浸泡1μg/g的EGF,和4)用10μg/gEGF浸泡的CM(每组n=5)。在KT增强后三个月处死实验动物。临床,组织学，并进行了组织形态计量学分析。
结果：FGG组临床KT区最高(5.16±1.63mm)。组织学上,所有组均出现颊骨裂开。关于新成立的KT，组间没有发现具体差异，但在FGG组的一些标本中形成了坚固的网钉。组织形态KT高度(4.66±1.81mm)和长度(5.56±2.25mm)在FGG组最高,而其余的则有类似的增加。在所有组中，植入物冠状部分的颊软组织厚度均不超过2mm。
结论：所有组的KT区增加，但FGG治疗更受青睐。向CM中添加EGF似乎不增强KT形成。
结论：FGG治疗比其他治疗方式更有利于重建KT区。
