关键词: Douglas-fir transcriptome Heartwood formation Inner sapwood Outer sapwood Seasonality Transition zone Wood tissues

Mesh : Transcriptome / genetics Pseudotsuga / genetics Plant Breeding Gene Expression Profiling Wood / genetics

来  源:   DOI:10.1186/s12863-023-01172-z   PDF(Pubmed)

Abstract:
Molecular cues linked to heartwood formation open new (complementary) perspectives to genetic breeding programs of Douglas-fir, a tree species largely cultivated in Europe for the natural durability and civil engineering properties of its wood.
RNAs from a single genotype of Douglas-fir, extracted from three distinct wood zones (outer sapwood, inner sapwood and transition zone) at four vegetative seasons to generate an extensive RNA-seq dataset used to apprehend the in-wood dynamic and seasonality of heartwood formation in this hardwood model species. Previously published data collected on somatic embryos of the same genotype could be merged with the present dataset to upgrade grade the Douglas-fir reference transcriptome.
摘要:
目标:与心材形成相关的分子线索为道格拉斯冷杉的遗传育种计划开辟了新的(互补的)观点,在欧洲种植的一种树种,其木材的自然耐久性和土木工程特性。
方法:来自道格拉斯冷杉单一基因型的RNA,从三个不同的木材区域(外部边材,内部边材和过渡区)在四个营养季节生成广泛的RNA-seq数据集,用于了解该硬木模型物种中心材形成的木材动态和季节性。先前在相同基因型的体细胞胚胎上收集的数据可以与本数据集合并,以提高道格拉斯冷杉参考转录组的等级。
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