Abstract：
The aim of this study was twofold: (1) evaluate the effect of benzo[a]pyrene (BaP) on expression levels of AQP3 and Notch1 genes in HaCaT cells exposed \"in vitro\" and (2) investigate the possible biological role of assessed genes by bioinformatics methods. Cells were exposed to increasing concentrations of BaP (0.0-4.0 μM) for 1-4 days. After treatments, cell viability and expression levels of AhR, CYP1A1, AQP3, and Notch1 genes were evaluated. The possible biological role of assessed genes was evaluated using bioinformatics tools. Low cytotoxicity in HaCaT cells dosed with BaP was detected. A significant overexpression (p < .05) of CYP1A1, AQP3, and Notch1 was found in exposed HaCaT cells. The gene expression upregulation was dependent on AhR activation. The bioinformatics analysis showed that these genes were enriched in related cancer signaling pathways. The findings suggest that AQP3 and Notch1 are upregulated by AhR activation in HaCaT cells exposed to BaP.
摘要：
这项研究的目的是双重的：1）评估苯并[a]芘（BaP）对暴露于“体外”的HaCaT细胞中AQP3和Notch1基因表达水平的影响，以及2）通过生物信息学方法研究评估基因的可能生物学作用。将细胞暴露于递增浓度的BaP(0.0-4.0μM)1-4天。治疗后,细胞活力和AhR的表达水平，评价CYP1A1、AQP3和Notch1基因。使用生物信息学工具评估了评估基因的可能生物学作用。在用BaP给药的HaCaT细胞中检测到低细胞毒性。在暴露的HaCaT细胞中发现CYP1A1、AQP3和Notch1的显著过表达(p<0.05)。基因表达上调依赖于AhR激活。生物信息学分析表明，这些基因在相关的癌症信号通路中富集。研究结果表明，在暴露于BaP的HaCaT细胞中，AQP3和Notch1被AhR激活上调。本文受版权保护。保留所有权利。
