Abstract：
Organoids can meet the needs between the use of cell culture and in vivo work, bringing together aspects of multicellular tissues, providing a more similar in vitro system for the study of various components, including host-interactions with pathogens and drug response. Organoids are structures that resemble organs in vivo, originating from pluripotent stem cells (PSCs) or adult stem cells (ASCs). There is great interest in deepening the understanding of the use of this technology to produce information about fungal infections and their treatments. This work aims the use 2D human lung organoid derived from human embryonic stem cells (hESCs), to investigate Cryptococcus neoformans-host interactions. C. neoformans is an opportunistic fungus acquired by inhalation that causes systemic mycosis mainly in immunocompromised individuals. Our work highlights the suitability of human minilungs for the study of C. neoformans infection (adhesion, invasion and replication), the interaction with the surfactant and induction of the host\'s alveolar pro-inflammatory response.
摘要：
类器官可以满足使用细胞培养和体内工作之间的需求，汇集多细胞组织的各个方面，为各种成分的研究提供了一个更相似的体外系统，包括宿主与病原体的相互作用和药物反应。类器官是类似于体内器官的结构，源自多能干细胞(PSC)或成体干细胞(ASC)。人们非常有兴趣加深对使用该技术来产生有关真菌感染及其治疗的信息的理解。这项工作的目的是使用2D人类肺类器官来源于人类胚胎干细胞（hESCs），研究新生隐球菌与宿主的相互作用。新生梭菌是通过吸入获得的机会性真菌,其主要在免疫受损个体中引起全身性真菌病。我们的工作强调了人类小龙对新型梭菌感染研究的适用性(粘连，入侵和复制)，与表面活性剂的相互作用和诱导宿主的肺泡促炎反应。
